LOCUS       sequence-192676-        4398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  drives expression of kaiBC from an rpaA-independent promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: P0050::kaiBC
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(978..1793)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(978..1793)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1701..1720
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1785..1804
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 gtgctctcgc aaattgcaat ctgcatttgg gtggaatcga cggcaatttt gcaggattgc
       61 cagcgggcgc tgtcggccga tcgctatcaa ctccaagtct gtgagtctgg cgaaatgctc
      121 ttggagtatg cccaaaccca tcgtgaccaa atcgactgcc tgattttagt ggcagccaat
      181 cccagcttca gggcagttgt tcagcagctc tgctttgagg gagtggtggt accagcgatt
      241 gtcgtaggcg atcgcgacag tgaggatccc gatgaaccag ccaaagaaca gctctatcac
      301 agcgctgaac tgcacctcgg tatccatcag ctcgagcaat tgccctacca agttgatgct
      361 gcactggctg aatttctgcg cttagccccg gtcgagacca tggccgacca catcatgctg
      421 atgggggcca accacgatcc cgagctatcg agccagcagc gggacctcgc tcagcgacta
      481 caagagcgcc taggctatct cggggtctac tacaagcgtg atcccgatcg ctttctgcgc
      541 aacctacccg cctacgaaag ccaaaagctg caccaagcga tgcagactag ctatcgtgaa
      601 atcgttttga gctatttttc gccgaatagc aacctcaacc agagcattga caacttcgtc
      661 aacatggctt tctttgccga tgttccagtc accaaagtgg tagaaattca catggagctg
      721 atggacgagt ttgccaagaa gctccgcgta gagggacgtt cagaggacat tttgctggat
      781 tatcggctga ctttaattga tgtaattgca catctttgtg agatgtatcg acggtctatc
      841 ccacgagaaa cctgaaaagg taaaggaggt cttaagctcg gctcaatttc tctctttatc
      901 ctgttagatg gtttgattgc tgttgctacc ccgttgatct gcgtctgcca gtgttacaac
      961 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     1021 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     1081 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     1141 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     1201 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     1261 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     1321 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     1381 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     1441 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     1501 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     1561 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     1621 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     1681 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     1741 ttggaattta atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc
     1801 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     1861 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     1921 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagggcc gcgctgcctt
     1981 tggttgcaat aacggtaaat ttaccgtctg tcccgattgt gccagtcacg ttaatattcg
     2041 ctgctttacc acttccagca gccgttactt ggctagcatt gccgcaagtt atgttaaagt
     2101 tacctgcact gctaccaata ccaagctgtg tacaagacgc attggtgacg tagttaggat
     2161 ctagtgtcgc cgcactaagg gtacgaagag cagcaccgac tgctgatttg ccttcagagg
     2221 cataggcttt atctgtttga tccaacaggt tcggtaatgc aactgctgcc aagatgccaa
     2281 cgataatgat aacgaccagc aactcgacaa gggtaaaacc tttttcttct tcaccttgtt
     2341 cgtctttacg ctgggcaaga tattgtagat aacgaagacg gagggagtca gacattcagg
     2401 agatcctcag caagagagct gttcttgaag taacttaccc ctacagcagg cttttatttc
     2461 aggttcctga aaatttccgc atgagccctc gtaaaaccta cattctcaag ctctacgtcg
     2521 ccggcaatac tccaaactca gtccgtgccc tcaaaacgct caagaacatt ctcgaagttg
     2581 aatttcaagg tgtttatgct ctaaaggtga tcgatgttct caaaaatcct cagttggcag
     2641 aagaggataa aatcctagcg acgccaaccc tcgccaaggt tctaccactg cctgtccgac
     2701 ggattattgg tgatttatcc gaccgtgaga aagttttgat tggccttgat ttactctacg
     2761 gcgaacttca agattccgac gacttctaac tgtcgttaac tgctttgttg gtactacctg
     2821 acttcaccct cttttaagat gacttccgct gagatgacta gccctaataa taattctgag
     2881 caccaagcca tcgctaagat gcgcacgatg attgaaggct ttgatgatat tagtcatggc
     2941 ggtcttccaa tcgggcgatc gaccctcgtt agtggtactt caggaaccgg caagaccctt
     3001 ttttctattc aatttctcta taacggtatt atcgagtttg atgagcctgg ggttttcgtt
     3061 actttcgaag aaaccccgca agatatcatt aaaaacgccc gtagttttgg ctgggattta
     3121 gccaagctgg tcgatgaggg caaactattt attcttgatg cttcacccga tccagaaggt
     3181 caagaggttg ttggcggctt cgatctctct gctctgattg agcggattaa ttatgcaatt
     3241 caaaagtatc gagcgcggcg ggtttcaatt gactcggtca cgtccgtttt ccagcaatat
     3301 gatgcctctt ctgtggttcg ccgcgaactc tttcggttgg tagctcgcct aaaacaaatt
     3361 ggggcaacta cggtcatgac caccgagcgt atcgaggaat atggcccgat cgctcgttac
     3421 ggtgttgagg aatttgtctc cgataacgtc gtgattctcc gcaacgtttt ggaaggggag
     3481 cgccgtcgcc gcaccctcga aatcctcaag ctacgtggca ccagccacat gaaaggggaa
     3541 tatccgttca cgattacgga tcatggcatc aatatcttcc cgctcggggc aatgcgcctt
     3601 acgcagcgat cgtcgaacgt gcgtgtttca tctggtgtcg tccgactcga tgaaatgtgt
     3661 ggtgggggct tctttaagga ctcaatcatt ctggcaactg gcgctacagg cactggtaaa
     3721 actctgttag ttagccgttt cgttgagaat gcttgtgcta acaaagagcg ggcgattctg
     3781 ttcgcttatg aagagtcacg agctcagctg ctccgcaatg cctattcatg gggaatggac
     3841 tttgaggaga tggagcgcca aaacctcctc aaaattgttt gcgcctatcc tgaatctgca
     3901 ggtcttgaag accatttgca gattattaag tcggagatca atgactttaa gccagctcgt
     3961 attgcaatcg actccctctc tgctttggcg cggggcgtta gcaacaatgc cttccgccaa
     4021 tttgtaattg gtgtcactgg ctacgcgaaa caagaagaaa tcacgggact attcacaaat
     4081 accagtgatc aatttatggg agcgcattcg attactgact cccatatctc aacaattacg
     4141 gatacgatta tcttgctcca atacgtcgag attcgtggcg aaatgtcccg cgccattaac
     4201 gtcttcaaga tgcgcggatc ttggcatgac aaagcaatcc gcgaattcat gatcagcgac
     4261 aaagggccgg acatcaagga ttctttccgg aactttgagc ggattatttc aggttcgcca
     4321 acacggatta ccgtcgatga gaaaagcgaa ctctcgcgaa ttgtgcgcgg cgttcaagaa
     4381 aaagggccgg agagctag
//
