LOCUS       sequence-192681-        3120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  drives expression of rpaA D53A variant from an rpaA-independent 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Puszynska AM, O'Shea EK
  TITLE     Switching of metabolic programs in response to light availability is
            an essential function of the cyanobacterial circadian output 
            pathway.
  JOURNAL   Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210.
  PUBMED    28430105
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-04-21"; volume: "6. pii: e23210. doi"; pages: " 
            10.7554/eLife.23210"
COMMENT     Sequence Label: P0050::rpaAD53A
FEATURES             Location/Qualifiers
     source          1..3120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             95..106
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(1039..1044,1045..1061,1062..1067)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1256..1789
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 cctcctagaa gctcatcagc gagatctctg gcaacaggtc agtccggcac tccttgatca
       61 actgcgatcg ctttcccttg aagcagaagc tgatattgaa ggtcgactag gaagtattcc
      121 agataacaag ttgcgctagt tggactttat tacaagcagt tgcaatcttc ggacgattct
      181 gctgtcgctg gttaccgtag aactattcct aatgcttgct caatccctat gagttcactt
      241 gttgttgtcg gatttcccaa ttcgcaagaa gccgaagaag tccgaatcaa acttgctcag
      301 ctacagacag aacatttaat tgccctagaa gatgctgttg ttgtcagcaa ggattccgag
      361 ggccatgtca aactcaatca agcgattaat ttaacggcaa ctggtgccgt cagcggcggt
      421 ttttggggca cccttgtcgg cttgatcttc ctcaatccct tggcgggtgc ggcgatcggt
      481 gcagcggctg gtgcactcag cggttccttg agcgacattg gcatcaacga tgactttctg
      541 cgcgaggtcg gcgaaaccct gcaggtcgga tcagccgccc tctgcctcct catccgcgaa
      601 gcaacccccg atcgcatcct gaaagagttg cgagtctacg ccaaagacgc ccggattcta
      661 caaacgaact tgagctttgc cgatgaagaa aacctcaagg cagccctaga aaaagccaaa
      721 gccgaagccc aagccctgcg tctcaattaa tctccaacgc ttcgatcgct cctgattact
      781 tgagttgatc aaggctcaga tcaagagcga tcgctacttc aatacagctt acttttggcc
      841 gctatctcaa cagcagttca atcctaagaa actgcagaag cagggattga gtaatttgat
      901 ggttgactgc agtccttatc agttgcttct caacaattat ccaaccgcaa ttacggacag
      961 agcactggac tagcaccact gctaaaaccg caaagctcgg gacgcacacc gtggaaacgg
     1021 atgaaggcac gaacccagtt gacataagcc tgttcggttc gtaaactgta atgcaagtag
     1081 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1141 gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc tcgggcatcc
     1201 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     1261 acgcagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc
     1321 aagtatgggc atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc
     1381 tgctcttgat cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc
     1441 ggactccgat tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt
     1501 cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt ctgcccaggt ttgagcagcc
     1561 gcgtagtgag atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat
     1621 tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat
     1681 ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat
     1741 acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca
     1801 agccgagatc ggcttcccgg ccgcggagtt gttcggtaaa ttgtcacaac gccgcactag
     1861 tggctgcctt tggttgcaat aacggtaaat ttaccgtctg tcccgattgt gccagtcacg
     1921 ttaatattcg ctgctttacc acttccagca gccgttactt ggctagcatt gccgcaagtt
     1981 atgttaaagt tacctgcact gctaccaata ccaagctgtg tacaagacgc attggtgacg
     2041 tagttaggat ctagtgtcgc cgcactaagg gtacgaagag cagcaccgac tgctgatttg
     2101 ccttcagagg cataggcttt atctgtttga tccaacaggt tcggtaatgc aactgctgcc
     2161 aagatgccaa cgataatgat aacgaccagc aactcgacaa gggtaaaacc tttttcttct
     2221 tcaccttgtt cgtctttacg ctgggcaaga tattgtagat aacgaagacg gagggagtca
     2281 gacattcagg agatcctcag caagagagct gttcttgaag taacttaccc ctacagcagg
     2341 cttttatttc aggttcctga aaatttccgc atgaaacccc gcatcctcgt gatcgatgat
     2401 gactcagcca tcttggagct ggtcgccgtc aatctggaga tgtctggcta tgacgtacgc
     2461 aaagctgagg acggcattaa aggtcaggct ttagctgttc agctagttcc cgacctgatc
     2521 atgctggctc taatgctgcc gcgggttgat ggctttaccg tctgtcagcg actgcggcgc
     2581 gatgagcgta ctgccgaaat tccggtgctg atgctgaccg ccctcggaca gactcaggat
     2641 aaggttgaag gcttcaacgc gggtgctgac gattatctga ctaagccctt cgaagttgaa
     2701 gagatgctgg cccgcgtgcg tgccttgctg cagcgcaccg atcgcattcc ccatgcagcc
     2761 cgccatagcg aaattctcag ctacggtccg ctgaccctga ttcccgagcg gtttgaggcc
     2821 atttggttca accgcacggt caagctgact cacttggaat ttgagttgtt gcactgcctg
     2881 ttgcaacgcc acggccaaac ggttgcgccg agcgaaatcc tcaaagaagt ctggggctat
     2941 gatcccgacg atgacatcga gacgattcgc gtccacatcc gtcatctgcg caccaagctc
     3001 gagcccgatc cccggcaccc gcgctacatc aaaacggtct atggagcggg ctactgcctt
     3061 gagctgccgg ccgagacgga actccaccaa cacgccgatc agtttccttc ggcgtcctga
//
