LOCUS       sequence-19269-e         551 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf192
FEATURES             Location/Qualifiers
     source          1..551
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             317..358
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             368..385
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(408..425)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    414..525
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 accatgattg tcgcatggtt tctattatta attttattgg agaaacacgg tcttccaatg
       61 acacaaggtt ctgtcaactt tttaatattg tcagatctaa ttttctttga tccactacag
      121 gcaaaatcag gtagttcgct acagaataca gatcttttca aatgcatgtt agcgataaat
      181 ggattaggtc ctttatccaa tacatctgtt aaaattgttt gagcaattcc cggggatccg
      241 aataaagggt caagacaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
      301 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
      361 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
      421 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      481 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      541 gtgtcattct a
//
