LOCUS       sequence-192705-        2936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit part-entry vector position 13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1707 HC_Kan_RFP-p13
FEATURES             Location/Qualifiers
     source          1..2936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    69..89
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(96..123)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(139..210)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(255..932)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             939..950
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    968..984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(992..998,999..1016,1017..1022))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1037..1058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1159..1180
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1264..1295
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1296..1398
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1399..2214
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1472..1491)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2235..2262
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2274..2861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2354..2373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2883..2912)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 cgctggagtc tgaggctcgt cctgaatgat atcaagcttg aattcgtttt cgtctcggta
       61 gagagaccct gcagcggccg ctactagtat ataaacgcag aaaggcccac ccgaaggtga
      121 gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      181 gtctttcgac tgagcctttc gttttatttg atgcctggct ctagtagcga tctacactag
      241 cactatcagc gttattaagc accggtggag tgacgacctt cagcacgttc gtactgttca
      301 acgatggtgt agtcttcgtt gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca
      361 cccggcagct gaaccggttt tttagccatg taggtggttt taacttcagc gtcgtagtga
      421 ccaccgtctt tcagtttcag acgcattttg atttcacctt tcagagcacc gtcttccggg
      481 tacatacgtt cggtggaagc ttcccaaccc atggtttttt tctgcataac cggaccgtcg
      541 gacgggaagt tggtaccacg cagtttaact ttgtagatga actcaccgtc ttgcagggag
      601 gagtcctggg taacggtaac aacaccaccg tcttcgaagt tcataacacg ttcccatttg
      661 aaaccttccg ggaaggacag tttcaggtag tccgggatgt cagccgggtg tttaacgtaa
      721 gctttggaac cgtactggaa ctgcggggac aggatgtccc aagcgaacgg cagcggacca
      781 cctttggtaa ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca
      841 ccttcgattt cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg
      901 aactctttga taacgtcttc ggaggaagcc atctagtatt tctcctcttt ctctagtatg
      961 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1021 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1081 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1141 gaggcggttt gcgtattgct ctagaagcgg ccgcgaattc ggtctctgtg acgagacgtg
     1201 acgaattctc tagatatcgc tcaatactga ccatttaaat catacctgac ctccatagca
     1261 gaaagtcaaa agcctccgac cggaggcttt tgacttgatc ggcacgtaag aggttccaac
     1321 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     1381 ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctc gaggccgcga
     1441 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1501 caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg
     1561 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag gctaaactgg
     1621 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1681 tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct
     1741 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     1801 cctgtttgta attgtccttt taacggcgat cgcgtatttc gtctcgctca ggcgcaatca
     1861 cgaatgaata acggtttggt tggtgcgagt gattttgatg acgagcgtaa tggctggcct
     1921 gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc
     1981 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2041 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2101 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2161 aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctgagggccc
     2221 aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat
     2281 aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac
     2341 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2401 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2461 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2521 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2581 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2641 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2701 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa
     2761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct
     2881 accgaagaaa ggcccacccg tgaaggtgag ccagtgagtt gattgcagtc cagtta
//
