LOCUS       sequence-192710-        7857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Gateway-compatible expression vector backbone with a 
            C-terminal Nanoluc luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7857)
  TITLE     Taipale Lab plasmids
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-ccdB-Nanoluc
FEATURES             Location/Qualifiers
     source          1..7857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     243..262
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             379..1404
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             379..1404
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    1534..1655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1571..1590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1625..1644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1704..1720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1704..1720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1717..1739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1728..1744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1752..1758,1759..1776,1777..1782))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1797..1818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1935..1952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2106..2694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2186..2205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2865..3656,3657..3725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3488..3507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3726..3830)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3905..3924)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4096..4475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4476..4679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4630..4650
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4724..4743
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4724..4742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4830..4954
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4979..4984,4985..5002,5003..5009)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5063..5722
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5129..5148)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6064..6369
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6248..6267
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6410..6534)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6602..7114
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(7140..7157)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7146..7370
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7416..7844
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7503..7522)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7713..7734
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(7853..7857,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
      361 ggatctgatc agcacgtgat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
      421 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
      481 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
      541 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
      601 ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt
      661 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
      721 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
      781 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
      841 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
      901 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
      961 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     1021 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     1081 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     1141 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     1201 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     1261 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     1321 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     1381 actcgtccga gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc
     1441 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     1501 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     1561 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1621 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     1681 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1741 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1801 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1861 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1921 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1981 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2041 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2101 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2161 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2221 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2281 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2341 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2401 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2461 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2521 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2581 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2641 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2761 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2821 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2881 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2941 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3001 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3061 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3121 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3181 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3241 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3301 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3361 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3421 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3481 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3541 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3601 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3661 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3721 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3841 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     3901 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     3961 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     4021 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     4081 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     4141 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4201 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4261 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     4321 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     4381 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     4441 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     4501 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     4561 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     4621 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     4681 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
     4741 gggagaccca agctggctag cgtttaaact taagcttggt accgagctcg gatccactag
     4801 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagct gaacgagaaa
     4861 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     4921 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     4981 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     5041 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     5101 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     5161 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     5221 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     5281 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     5341 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     5401 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     5461 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     5521 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     5581 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     5641 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     5701 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     5761 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     5821 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     5881 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     5941 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     6001 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     6061 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     6121 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     6181 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     6241 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     6301 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     6361 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     6421 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     6481 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgatat
     6541 ccagcacagt ggcggccgct cgagtggctc gggctcgacc tcgggctcgg gcaaaaccgg
     6601 tgtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga
     6661 ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac
     6721 tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat
     6781 catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt
     6841 ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat
     6901 cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt
     6961 gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga
     7021 cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac
     7081 cggctggcgg ctgtgcgaac gcattctggc gtaagggccc gtttaaaccc gctgatcagc
     7141 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     7201 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     7261 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     7321 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     7381 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     7441 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     7501 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     7561 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     7621 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     7681 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     7741 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     7801 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaat
//
