LOCUS       sequence-192736-        2935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit part-entry vector position 23.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1715 HC_Kan_RFP-p23
FEATURES             Location/Qualifiers
     source          1..2935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..59
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      complement(66..93)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(109..180)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(225..902)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             909..920
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    938..954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(962..968,969..986,987..992))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1007..1028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    1129..1150
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1234..1265
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1266..1368
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1369..2184
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1442..1461)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2205..2232
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2244..2831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2324..2343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2853..2882)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tatcaagctt gaattcgttt cgtctcgcgt gagagaccct gcagcggccg ctactagtat
       61 ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac
      121 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      181 atgcctggct ctagtagcga tctacactag cactatcagc gttattaagc accggtggag
      241 tgacgacctt cagcacgttc gtactgttca acgatggtgt agtcttcgtt gtgggaggtg
      301 atgtccagtt tgatgtcggt tttgtaagca cccggcagct gaaccggttt tttagccatg
      361 taggtggttt taacttcagc gtcgtagtga ccaccgtctt tcagtttcag acgcattttg
      421 atttcacctt tcagagcacc gtcttccggg tacatacgtt cggtggaagc ttcccaaccc
      481 atggtttttt tctgcataac cggaccgtcg gacgggaagt tggtaccacg cagtttaact
      541 ttgtagatga actcaccgtc ttgcagggag gagtcctggg taacggtaac aacaccaccg
      601 tcttcgaagt tcataacacg ttcccatttg aaaccttccg ggaaggacag tttcaggtag
      661 tccgggatgt cagccgggtg tttaacgtaa gctttggaac cgtactggaa ctgcggggac
      721 aggatgtccc aagcgaacgg cagcggacca cctttggtaa ctttcagttt agcggtctgg
      781 gtaccttcgt acggacgacc ttcaccttca ccttcgattt cgaactcgtg accgttaacg
      841 gaaccttcca tacgaacttt gaaacgcatg aactctttga taacgtcttc ggaggaagcc
      901 atctagtatt tctcctcttt ctctagtatg tgtgaaattg ttatccgctc acaattccac
      961 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1021 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1081 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattgct ctagaagcgg
     1141 ccgcgaattc ggtctctttc gcgagacgtg acgaattctc tagatatcgc tcaatactga
     1201 ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac cggaggcttt
     1261 tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     1321 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat gagccatatt
     1381 caacgggaaa cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1441 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat
     1501 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     1561 gttacagatg agatggtcag gctaaactgg ctgacggaat ttatgcctct tccgaccatc
     1621 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat cccagggaaa
     1681 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     1741 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacggcgat
     1801 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tggtgcgagt
     1861 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag
     1921 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     1981 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2041 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2101 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcac
     2161 ttgatgctcg atgagttttt ctgagggccc aaatgtaatc acctggctca ccttcgggtg
     2221 ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2281 aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2341 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2401 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2461 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2521 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2581 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2641 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2701 tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac
     2761 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2821 aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg tgaaggtgag
     2881 ccagtgagtt gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatga
//
