LOCUS       sequence-192744-        2720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit receiver vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3736_HC_Amp_ccdB_receiver vector
FEATURES             Location/Qualifiers
     source          1..2720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      29..60
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        61..163
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(164..232,233..1024)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(382..401)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1048..1075
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1087..1674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1167..1186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1696..1725)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(1829..1856)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1872..1943)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1984..2289)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2086..2105)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 taaatcatac ctgacctcca tagcagaaag tcaaaagcct ccgaccggag gcttttgact
       61 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      121 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatgagta ttcaacattt
      181 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      241 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      301 actggatctc aacagcggta agatccttga gagtttacgc cccgaagaac gttttccaat
      361 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      421 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      481 cacagaaaag catctcacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      541 catgagtgat aacactgcgg ccaacttact tctggcaacg atcggaggac cgaaggagct
      601 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      661 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      721 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      781 agactggatg gaggcggata aagttgcagg atcacttctg cgctcggccc tcccggctgg
      841 ctggtttatt gctgataaat ctggagccgg tgagcgtggt tctcgcggta tcattgcagc
      901 actggggcca gatggtaagc cctcccgcat cgtagttatc tacacgacgg ggagtcaggc
      961 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1021 gtaatgaggg cccaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct
     1081 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca
     1141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1501 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1561 agttacctcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1621 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1681 cctttgattt tctaccgaag aaaggcccac ccgtgaaggt gagccagtga gttgattgca
     1741 gtccagttac gctggagtct gaggctcgtc ctgaatgata tcaagcttga attcgttatc
     1801 gttgagacgg ccgctactag tatataaacg cagaaaggcc cacccgaagg tgagccagtg
     1861 tgactctagt agagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     1921 gactgagcct ttcgttttat ttgatgcctg gctctagtag cgatctacac tagcactatc
     1981 agcttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
     2041 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
     2101 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc
     2161 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
     2221 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
     2281 aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
     2341 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac
     2401 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt
     2461 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
     2521 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
     2581 cgcaaatacg catactgtta tctggctttt agtaagccgg atccctgcat taatgaatcg
     2641 gccaacgcgc ggggagaggc ggtttgcgta ttgcgtctct cctatacgaa ttctctagat
     2701 atcgctcaat actgaccatt
//
