LOCUS       sequence-192746-        1847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector B-C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3732 HC_Kan_conn_B-C
FEATURES             Location/Qualifiers
     source          1..1847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      127..158
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        159..261
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             262..1077
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(335..354)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1098..1125
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1137..1724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1217..1236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1746..1775)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 acgtctcgat ggactaacta cggtgaaaga gacttagaag gagattgaga gagactcgag
       61 acgacgaatt ctctagatat cgctcaatac tgaccattta aatcatacct gacctccata
      121 gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt aagaggttcc
      181 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
      241 tcaggagcta aggaagctaa aatgagccat attcaacggg aaacgtcttg ctcgaggccg
      301 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      361 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
      421 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt caggctaaac
      481 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
      541 gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat
      601 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
      661 attcctgttt gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc tcaggcgcaa
      721 tcacgaatga ataacggttt ggttggtgcg agtgattttg atgacgagcg taatggctgg
      781 cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc
      841 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
      901 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
      961 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1021 gataatcctg atatgaataa attgcagttt cacttgatgc tcgatgagtt tttctgaggg
     1081 cccaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc
     1141 cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga
     1201 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1261 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1321 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1381 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1441 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1501 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1561 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg
     1621 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1681 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt
     1741 tctaccgaag aaaggcccac ccgtgaaggt gagccagtga gttgattgca gtccagttac
     1801 gctggagtct gaggctcgtc ctgaatgata tcaagcttga attcgtt
//
