LOCUS       sequence-19275-e         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf198
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             578..619
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(669..686)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gtacccttca ccgaattcca gctgaccacc atggctaagc gagtaagcct tccagatgtg
       61 gttatttcag cacctaaagc agtctttaag cccgctaaag aagaagcact cgcttgtata
      121 ctaccaaagt attataaatc tatggcagat gtgtctatta agacaaatag tgtaattgat
      181 aagtgttggt tttgtaatca agatttggtt tttaaaccta ttagtattga gacattcaag
      241 ggtggtgaag ttgggtattt ctgttctaaa atatgtaggg attcgttggc gtctatggtt
      301 aagtctcacg tagctcttag agaagaacca aaaatttctt tgttgccttt agtattctat
      361 gaagataagg aaaaggttat aaatacaata aacttactaa gagataaaga cggcgtttac
      421 ggaagctgtt actttaagga aaactcacaa attatagata tttctctacg gagtttattg
      481 taagcaattc ccggggatcc gaataaaggg tcaagacaat tctgcagata tccagcacag
      541 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
      601 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
      661 ctgatcagcc tcgactgtgc cttctagttg
//
