LOCUS       sequence-192750-        1846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector E-F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3756 HC_Kan_conn_E-F
FEATURES             Location/Qualifiers
     source          1..1846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      99..130
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        131..233
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             234..1049
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(307..326)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1070..1097
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1109..1696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1189..1208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1718..1747)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ccgtcagaat taattcagat cgatctacca gcgagacgaa ttctctagat atcgctcaat
       61 actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct ccgaccggag
      121 gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
      181 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatgagcc
      241 atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt
      301 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
      361 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
      421 atgatgttac agatgagatg gtcaggctaa actggctgac ggaatttatg cctcttccga
      481 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatcccag
      541 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
      601 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaacg
      661 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttggtg
      721 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
      781 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
      841 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
      901 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
      961 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     1021 ttcacttgat gctcgatgag tttttctgag ggcccaaatg taatcacctg gctcaccttc
     1081 gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1141 acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1561 gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg
     1621 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1681 aaaaaaagga tctcaagaag atcctttgat tttctaccga agaaaggccc acccgtgaag
     1741 gtgagccagt gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga
     1801 tatcaagctt gaattcgtta cgtctcgtcc gttagccttt agtgaa
//
