LOCUS       sequence-192752-       10296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10296)
  AUTHORS   Kao WH, Beaudenon SL, Talis AL, Huibregtse JM, Howley PM
  TITLE     Human papillomavirus type 16 E6 induces self-ubiquitination of the 
            E6AP ubiquitin-protein ligase.
  JOURNAL   J Virol 2000 Jul;74(14):6408-17.
  PUBMED    10864652
REFERENCE   2  (bases 1 to 10296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol"; 
            date: "2000-07"; volume: "74(14)"; pages: "6408-17"
COMMENT     Sequence Label: p4054 HA-E6AP isoform II
FEATURES             Location/Qualifiers
     source          1..10296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        178..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        558..761
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     712..732
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             838..864
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3530..4152
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3729..3748)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4176..4196)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4181..4510
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4361..4496
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4549..4645
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(4722..4740)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4754..4770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4767..4789)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4778..4794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4802..4808,4809..4826,4827..4832))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4847..4868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5156..5744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5837..6628)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     7059..7075
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7080..7098
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7116..7215
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(7220..7319)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7335..7351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      7415..7501
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7593..7620
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(7950..8538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8709..9500,9501..9569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9332..9351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9570..9674)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9701..10156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9788..9807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9998..10019
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca
       61 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
      121 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
      181 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
      601 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      661 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
      721 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      781 attgttttta tttttaattt tctttcaaat acttccatcg aattcagatc caccatgtac
      841 ccatacgatg ttccagatta cgctgagaag ctgcaccagt gttattggaa atcaggagaa
      901 cctcagtctg acgacattga agctagccga atgaagcgag cagctgcaaa gcatctaata
      961 gaacgctact accaccagtt aactgagggc tgtggaaatg aagcctgcac gaatgagttt
     1021 tgtgcttcct gtccaacttt tcttcgtatg gataataatg cagcagctat taaagccctc
     1081 gagctttata agattaatgc aaaactctgt gatcctcatc cctccaagaa aggagcaagc
     1141 tcagcttacc ttgagaactc gaaaggtgcc cccaacaact cctgctctga gataaaaatg
     1201 aacaagaaag gcgctagaat tgattttaaa gatgtgactt acttaacaga agagaaggta
     1261 tatgaaattc ttgaattatg tagagaaaga gaggattatt cccctttaat ccgtgttatt
     1321 ggaagagttt tttctagtgc tgaggcattg gtacagagct tccggaaagt taaacaacac
     1381 accaaggaag aactgaaatc tcttcaagca aaagatgaag acaaagatga agatgaaaag
     1441 gaaaaagctg catgttctgc tgctgctatg gaagaagact cagaagcatc ttcctcaagg
     1501 ataggtgata gctcacaggg agacaacaat ttgcaaaaat taggccctga tgatgtgtct
     1561 gtggatattg atgccattag aagggtctac accagattgc tctctaatga aaaaattgaa
     1621 actgcctttc tcaatgcact tgtatatttg tcacctaacg tggaatgtga cttgacgtat
     1681 cacaatgtat actctcgaga tcctaattat ctgaatttgt tcattatcgt aatggagaat
     1741 agaaatctcc acagtcctga atatctggaa atggctttgc cattattttg caaagcgatg
     1801 agcaagctac cccttgcagc ccaaggaaaa ctgatcagac tgtggtctaa atacaatgca
     1861 gaccagattc ggagaatgat ggagacattt cagcaactta ttacttataa agtcataagc
     1921 aatgaattta acagtcgaaa tctagtgaat gatgatgatg ccattgttgc tgcttcgaag
     1981 tgcttgaaaa tggtttacta tgcaaatgta gtgggagggg aagtggacac aaatcacaat
     2041 gaagaagatg atgaagagcc catccctgag tccagcgagc tgacacttca ggaacttttg
     2101 ggagaagaaa gaagaaacaa gaaaggtcct cgagtggacc ccctggaaac tgaacttggt
     2161 gttaaaaccc tggattgtcg aaaaccactt atcccttttg aagagtttat taatgaacca
     2221 ctgaatgagg ttctagaaat ggataaagat tatacttttt tcaaagtaga aacagagaac
     2281 aaattctctt ttatgacatg tccctttata ttgaatgctg tcacaaagaa tttgggatta
     2341 tattatgaca atagaattcg catgtacagt gaacgaagaa tcactgttct ctacagctta
     2401 gttcaaggac agcagttgaa tccatatttg agactcaaag ttagacgtga ccatatcata
     2461 gatgatgcac ttgtccggct agagatgatc gctatggaaa atcctgcaga cttgaagaag
     2521 cagttgtatg tggaatttga aggagaacaa ggagttgatg agggaggtgt ttccaaagaa
     2581 ttttttcagc tggttgtgga ggaaatcttc aatccagata ttggtatgtt cacatacgat
     2641 gaatctacaa aattgttttg gtttaatcca tcttcttttg aaactgaggg tcagtttact
     2701 ctgattggca tagtactggg tctggctatt tacaataact gtatactgga tgtacatttt
     2761 cccatggttg tctacaggaa gctaatgggg aaaaaaggaa cttttcgtga cttgggagac
     2821 tctcacccag ttctatatca gagtttaaaa gatttattgg agtatgaagg gaatgtggaa
     2881 gatgacatga tgatcacttt ccagatatca cagacagatc tttttggtaa cccaatgatg
     2941 tatgatctaa aggaaaatgg tgataaaatt ccaattacaa atgaaaacag gaaggaattt
     3001 gtcaatcttt attctgacta cattctcaat aaatcagtag aaaaacagtt caaggctttt
     3061 cggagaggtt ttcatatggt gaccaatgaa tctcccttaa agtacttatt cagaccagaa
     3121 gaaattgaat tgcttatatg tggaagccgg aatctagatt tccaagcact agaagaaact
     3181 acagaatatg acggtggcta taccagggac tctgttctga ttagggagtt ctgggaaatc
     3241 gttcattcat ttacagatga acagaaaaga ctcttcttgc agtttacaac gggcacagac
     3301 agagcacctg tgggaggact aggaaaatta aagatgatta tagccaaaaa tggcccagac
     3361 acagaaaggt tacctacatc tcatacttgc tttaatgtgc ttttacttcc ggaatactca
     3421 agcaaagaaa aacttaaaga gagattgttg aaggccatca cgtatgccaa aggatttggc
     3481 atgctgtaaa acaagcttgc atgcctgcag gtcgactcta gaggatcccg ggtggcatcc
     3541 ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag
     3601 ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat
     3661 tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc
     3721 ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat
     3781 ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca
     3841 ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat
     3901 attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa
     3961 attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttaaaata
     4021 actataccag caggaggacg tccagacaca gcataggcta cctggccatg cccaaccggt
     4081 gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca ctcagtagat
     4141 gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt tagggtgtgg
     4201 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4261 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4321 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4381 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4441 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4501 cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaactggt aagtttagtc
     4561 tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag aactgctcct
     4621 cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc tctaaaagct
     4681 gctgcaggtc gactctaggg ccgcgtaccg agctcgaatt ccctatagtg agtcgtatta
     4741 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4801 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4861 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4921 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4981 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5041 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5101 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5161 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5221 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5281 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5341 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5401 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5461 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5521 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5581 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5641 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5701 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5761 tttctacggg gtctgacgct cagtggaacg acgcgtaact cacgttaagg gattttggtc
     5821 atgggtggct cgagggttat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
     5881 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
     5941 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
     6001 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
     6061 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
     6121 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
     6181 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
     6241 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
     6301 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
     6361 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa
     6421 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
     6481 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
     6541 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
     6601 atacttcggc gatcaccgct tccctcataa tgtttaactt tgttttaggg cgactgccct
     6661 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
     6721 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga
     6781 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     6841 agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc
     6901 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg
     6961 aggcatttct gtcctggctg gtctagagga ggcatgatga tatattttta tcttgtgcaa
     7021 tgtaacatca gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt
     7081 aatacgactc actatagggg atatcagctg gatggcaaat aatgatttta ttttgactga
     7141 tagtgacctg ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt
     7201 acaagaaagt tggtcatggt gccaactttt ttgtacaaag ttggcattat aaaaaagcat
     7261 tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg
     7321 ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa catccatgct
     7381 agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgga
     7441 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     7501 tccgccggga gcggatttga acgttgtgaa gcaacggccc ggagggtggc gggcaggacg
     7561 cccgccataa actgccaggc atcaaactaa gcagaaggcc atcctgacgg atggcctttt
     7621 tgcgtttcta caaactcttc ctggctagcg gtacgcgtat taattgcgtt gcgctcactg
     7681 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7741 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     7801 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7861 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7921 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7981 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8041 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8101 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8161 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8221 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8281 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8341 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8401 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8461 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8521 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8581 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8641 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8701 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8761 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8821 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8881 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8941 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9001 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9061 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9121 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9181 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9241 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9301 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9361 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9421 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9481 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9541 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9601 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9661 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     9721 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     9781 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     9841 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9901 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9961 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    10021 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10081 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10141 ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10201 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10261 gttgggtaac gccagggttt tcccagtcac gacgtt
//
