LOCUS       sequence-192759-        1896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector H-K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1921 HC_Kan_conn_H-K
FEATURES             Location/Qualifiers
     source          1..1896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      20..51
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        52..154
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             155..970
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(228..247)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      991..1018
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1030..1617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1110..1129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1639..1668)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca
       61 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
      121 gttatcgaga ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc
      181 ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
      241 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
      301 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
      361 ggtcaggcta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
      421 tactcctgat gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt
      481 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
      541 ccggttgcat tcgattcctg tttgtaattg tccttttaac ggcgatcgcg tatttcgtct
      601 cgctcaggcg caatcacgaa tgaataacgg tttggttggt gcgagtgatt ttgatgacga
      661 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc
      721 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
      781 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
      841 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
      901 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcacttga tgctcgatga
      961 gtttttctga gggcccaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt
     1021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag
     1081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1501 gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1621 gatcctttga ttttctaccg aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt
     1681 gcagtccagt tacgctggag tctgaggctc gtcctgaatg atatcaagct tgaattcgtt
     1741 gttatgctcg agggtgagta gcgtctcgta cccgaggata tcacctgtag ggatgttcta
     1801 gagtccggaa gctgttatta gccttgagac ggtgactaga tcgtggatgc agacgaattc
     1861 tctagatatc gctcaatact gaccatttaa atcata
//
