LOCUS       sequence-192760-        1877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector H-J.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2986 HC_Kan_conn_H-J
FEATURES             Location/Qualifiers
     source          1..1877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      222..253
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        254..356
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             357..1172
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(430..449)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1193..1220
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1232..1819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1312..1331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1841..1870)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttttaa cgcagccgtc tccaggctaa taacagcttc cggactctag aacatcccta
      121 caggtgatat cctcgtgctt gagacgaggc cgtttatgac gaattctcta gatatcgctc
      181 aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag cctccgaccg
      241 gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
      301 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatga
      361 gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg
      421 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
      481 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
      541 ccaatgatgt tacagatgag atggtcaggc taaactggct gacggaattt atgcctcttc
      601 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
      661 cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
      721 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
      781 acggcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
      841 gtgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
      901 tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
      961 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     1021 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     1081 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     1141 agtttcactt gatgctcgat gagtttttct gagggcccaa atgtaatcac ctggctcacc
     1201 ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1261 atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1321 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1681 ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta gctcttgatc
     1741 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1801 cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg cccacccgtg
     1861 aaggtgagcc agtgagt
//
