LOCUS       sequence-192762-        1871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector Ha-J.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3734 HC_Kan_conn_Ha-J
FEATURES             Location/Qualifiers
     source          1..1871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(97..116)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     terminator      216..247
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        248..350
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             351..1166
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(424..443)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1187..1214
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1226..1813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1306..1325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1835..1864)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttttaa cgcagccgtc tccatccgag gctaactgaa acacggaagg agacaataca
      121 ggaaggaact gcttgagacg aggccgttta tgacgaattc tctagatatc gctcaatact
      181 gaccatttaa atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct
      241 tttgacttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
      301 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata
      361 ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat
      421 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
      481 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      541 atgttacaga tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca
      601 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atcccaggga
      661 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
      721 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg
      781 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga
      841 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
      901 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
      961 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     1021 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     1081 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     1141 acttgatgct cgatgagttt ttctgagggc ccaaatgtaa tcacctggct caccttcggg
     1201 tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1261 aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1681 tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa
     1741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1801 aaaaggatct caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg
     1861 agccagtgag t
//
