LOCUS       sequence-192765-        1857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector H-Ha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1913 HC_Kan_conn_H-Ha
FEATURES             Location/Qualifiers
     source          1..1857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      27..58
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        59..161
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             162..977
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(235..254)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      998..1025
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1037..1624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1117..1136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1646..1675)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 aatcatacct gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg
       61 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
      121 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg
      181 aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
      241 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
      301 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
      361 atgagatggt caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
      421 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat
      481 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
      541 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat
      601 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgattttg
      661 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc
      721 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
      781 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
      841 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
      901 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt cacttgatgc
      961 tcgatgagtt tttctgaggg cccaaatgta atcacctggc tcaccttcgg gtgggccttt
     1021 ctgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat
     1081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1141 gaagctccct cgtgcgctct cctgttccga ccctgtcgct taccggatac ctgtccgcct
     1201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1441 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1501 tgctgaagcc agttacctcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1621 tcaagaagat cctttgattt tctaccgaag aaaggcccac ccgtgaaggt gagccagtga
     1681 gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata tcaagcttga
     1741 attcgtttcg tctcgggatc gaggatatca cctgtaggga tgttctagag tccggaagct
     1801 gttattagcc tcgagacgtg acgaattctc tagatatcgc tcaatactga ccattta
//
