LOCUS       sequence-192767-        1901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector L-M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3760 HC_Kan_conn_L-M
FEATURES             Location/Qualifiers
     source          1..1901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      181..212
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        213..315
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             316..1131
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(389..408)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1152..1179
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1191..1778)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1271..1290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1800..1829)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 acgtctcgcg tctatgatga ggatgttggt ggagagcatg tggaggaagt ggatagggaa
       61 ggttgtagag tagatccggt tgaagtgatg aggataggag gaggtcaccg agacgaaacg
      121 aattctctag atatcgctca atactgacca tttaaatcat acctgacctc catagcagaa
      181 agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt
      241 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
      301 gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta
      361 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
      421 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
      481 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg
      541 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
      601 ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat
      661 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
      721 gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
      781 atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
      841 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
      901 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
      961 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     1021 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     1081 cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttctg agggcccaaa
     1141 tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg
     1201 ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg
     1261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac ctcggaaaaa
     1681 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1741 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg attttctacc
     1801 gaagaaaggc ccacccgtga aggtgagcca gtgagttgat tgcagtccag ttacgctgga
     1861 gtctgaggct cgtcctgaat gatatcaagc ttgaattcgt t
//
