LOCUS       sequence-19277-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf200
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             352..393
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             403..420
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(443..460)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    449..673
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(806..825)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagctcggat ccgtaccctt cccgaattcc agctgaccac catgagttgg tacgaaaaat
       61 ataacattgt actgaatccg cctaagcggt gttcttttgc atgtgcggat aatttaacta
      121 ctatattggc ggaagacggt aacaatatta gggcgatact ttattcacag cccaaaaaac
      181 taaaaatatt acaggatttt ctggcaacgt ctagaaataa aatgttttta tataaaatat
      241 tggacgacga gatacgtaga gtgttaacat gagcaattcc cggggatccg tcgacctgca
      301 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
      361 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
      421 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      481 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      541 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      601 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      661 gtgggctcta tggcttctga ggcggagaga accagctggg gctctagggg gtatccccac
      721 gcgccctgta gcggcgcatt aatcgcggcg ggtgtggtgg ttacacgcag cgtgaccgct
      781 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctc
//
