LOCUS       sequence-192770-        1939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector L-Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1918 HC_Kan_conn_L-Y
FEATURES             Location/Qualifiers
     source          1..1939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      34..65
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        66..168
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             169..984
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(242..261)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1005..1032
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1044..1631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1124..1143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1653..1682)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac cggaggcttt
       61 tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
      121 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat gagccatatt
      181 caacgggaaa cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat
      241 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat
      301 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
      361 gttacagatg agatggtcag gctaaactgg ctgacggaat ttatgcctct tccgaccatc
      421 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat cccagggaaa
      481 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
      541 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacggcgat
      601 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tggtgcgagt
      661 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag
      721 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
      781 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
      841 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
      901 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcac
      961 ttgatgctcg atgagttttt ctgagggccc aaatgtaatc acctggctca ccttcgggtg
     1021 ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1081 aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1141 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1201 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1261 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1321 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1381 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1441 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1501 tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac
     1561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1621 aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg tgaaggtgag
     1681 ccagtgagtt gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca
     1741 agcttgaatt cgttagctca atctcctcat tcaccgtctc gcagtcctcc tcctatcctc
     1801 atcacttcaa ccggatctac tctacaacct tccctatcca cttcctccac atgctctcca
     1861 ccaacatcct catcatagac gtgagacgct ctgaactcac caacttctga cgaattctct
     1921 agatatcgct caatactga
//
