LOCUS       sequence-192771-        1921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector M-R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3733 HC_Kan_conn_M-R
FEATURES             Location/Qualifiers
     source          1..1921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      266..297
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        298..400
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             401..1216
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(474..493)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1237..1264
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1276..1863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1356..1375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1885..1914)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtttttaa cgcagccgtc tcctcacgac ataatccacc atcaacatgc aagtccagtc
      121 aacaactttc cctcagacgt tagtggtaat cgaacctcaa cacctagccc agaacaagcc
      181 ggttgagacg aggccgttta tgacgaattc tctagatatc gctcaatact gaccatttaa
      241 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga
      301 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      361 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga
      421 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      481 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
      541 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
      601 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
      661 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt
      721 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
      781 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt
      841 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga
      901 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
      961 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     1021 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     1081 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     1141 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct
     1201 cgatgagttt ttctgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc
     1261 tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg
     1321 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1381 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1441 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1501 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1561 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1621 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1681 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1741 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1861 caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag
     1921 t
//
