LOCUS       sequence-192773-        1895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector Q-R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2020 HC_Kan_conn_Q-R
FEATURES             Location/Qualifiers
     source          1..1895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      240..271
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        272..374
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             375..1190
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(448..467)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1211..1238
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1250..1837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1330..1349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1859..1888)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgtacgtct cggctcgaca taatccacca tcaacatgca agtccagtca acaactttcc
      121 ctcagacgtt agtggtaatc gaacctcaac acctagccca gaacaagccg gtcgagacga
      181 attctctaga tatcgctcaa tactgaccat ttaaatcata cctgacctcc atagcagaaa
      241 gtcaaaagcc tccgaccgga ggcttttgac ttgatcggca cgtaagaggt tccaactttc
      301 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
      361 ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa
      421 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
      481 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac
      541 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcaggcta aactggctga
      601 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
      661 tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa tatcctgatt
      721 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
      781 tttgtaattg tccttttaac ggcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
      841 tgaataacgg tttggttggt gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
      901 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc
      961 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     1021 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     1081 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     1141 ctgatatgaa taaattgcag tttcacttga tgctcgatga gtttttctga gggcccaaat
     1201 gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt ttccataggc
     1261 tccgcccccc tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga
     1321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag
     1741 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1801 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga ttttctaccg
     1861 aagaaaggcc cacccgtgaa ggtgagccag tgagt
//
