LOCUS       sequence-192777-        1845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector U-V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3757 HC_Kan_conn_U-V
FEATURES             Location/Qualifiers
     source          1..1845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..52
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        53..155
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             156..971
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(229..248)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      992..1019
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1031..1618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1111..1130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1640..1669)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 acctgacctc catagcagaa agtcaaaagc ctccgaccgg aggcttttga cttgatcggc
       61 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
      121 agttatcgag attttcagga gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt
      181 cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      241 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
      301 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
      361 tggtcaggct aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
      421 gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg
      481 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
      541 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc
      601 tcgctcaggc gcaatcacga atgaataacg gtttggttgg tgcgagtgat tttgatgacg
      661 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct
      721 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
      781 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
      841 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
      901 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcacttg atgctcgatg
      961 agtttttctg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt
     1021 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa
     1081 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1141 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1201 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1261 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1321 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1381 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1441 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1501 agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1621 agatcctttg attttctacc gaagaaaggc ccacccgtga aggtgagcca gtgagttgat
     1681 tgcagtccag ttacgctgga gtctgaggct cgtcctgaat gatatcaagc ttgaattcgt
     1741 ttcgtctcgc aactgtatgt gtggcgggtg ttatggggtt gttggattat ccacgagacg
     1801 tgacgaattc tctagatatc gctcaatact gaccatttaa atcat
//
