LOCUS       sequence-192779-        1940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector W-Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2733 HC_Kan_conn_W-Y
FEATURES             Location/Qualifiers
     source          1..1940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      28..59
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        60..162
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             163..978
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(236..255)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      999..1026
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1038..1625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1118..1137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1647..1676)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg cttttgactt
       61 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
      121 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca tattcaacgg
      181 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      241 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
      301 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      361 gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      421 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg gaaaacagca
      481 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
      541 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg cgatcgcgta
      601 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc gagtgatttt
      661 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
      721 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
      781 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
      841 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
      901 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcacttgatg
      961 ctcgatgagt ttttctgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt
     1021 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1081 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1141 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1201 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1261 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1321 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1381 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1441 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1501 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1621 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgaagg tgagccagtg
     1681 agttgattgc agtccagtta cgctggagtc tgaggctcgt cctgaatgat atcaagcttg
     1741 aattcgttac acgctttcat ccacctgcgt ctcgcagtgg gatgtgtgag tttgttgagt
     1801 gaatggtaat tgtgggagtg gtgtgggatt tgaggtgtgc tggtgtggtt gtgacgagga
     1861 tcaatatgtg ttcgtgagac gctgaagtgg acgttaggat agtgacgaat tctctagata
     1921 tcgctcaata ctgaccattt
//
