LOCUS       sequence-192780-        1901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector W-X.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3159 HC_Kan_conn_W-X
FEATURES             Location/Qualifiers
     source          1..1901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      30..61
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        62..164
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             165..980
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(238..257)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1001..1028
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1040..1627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1120..1139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1649..1678)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
       61 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
      121 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatgagc catattcaac
      181 gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      241 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
      301 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      361 cagatgagat ggtcaggcta aactggctga cggaatttat gcctcttccg accatcaagc
      421 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca gggaaaacag
      481 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
      541 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac ggcgatcgcg
      601 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttggt gcgagtgatt
      661 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
      721 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
      781 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
      841 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
      901 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcacttga
      961 tgctcgatga gtttttctga gggcccaaat gtaatcacct ggctcacctt cgggtgggcc
     1021 tttctgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1081 gatgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1501 ctctgctgaa gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1561 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1621 atctcaagaa gatcctttga ttttctaccg aagaaaggcc cacccgtgaa ggtgagccag
     1681 tgagttgatt gcagtccagt tacgctggag tctgaggctc gtcctgaatg atatcaagct
     1741 tgaattcgtt tcgtctcgcg tgcagataga caggaagaat cgacagaaca tccgacgaat
     1801 agaggaccac gggcagcaga agaccatgtc taaagtgcga gactgaatac aagattcgcg
     1861 agacgtgacg aattctctag atatcgctca atactgacca t
//
