LOCUS       sequence-192781-        1901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA toolkit Assembly connector Y-Z.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2066 HC_Kan_conn_Y-Z
FEATURES             Location/Qualifiers
     source          1..1901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      26..57
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        58..160
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             161..976
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(234..253)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      997..1024
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1036..1623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1116..1135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1645..1674)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga
       61 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
      121 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga
      181 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      241 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
      301 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
      361 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
      421 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt
      481 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
      541 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt
      601 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga
      661 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
      721 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
      781 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
      841 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
      901 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct
      961 cgatgagttt ttctgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc
     1021 tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg
     1081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1441 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1501 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1561 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1621 caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag
     1681 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa
     1741 ttcgtttcgt ctcgtcgtgg gatgtgtgag tttgttgagt gaatggtaat tgtgggagtg
     1801 gtgtgggatt tgaggtgtgc tggtgtggtt gtgacgagga tcaatatgtg cagtcgagac
     1861 gtgacgaatt ctctagatat cgctcaatac tgaccattta a
//
