LOCUS       sequence-192791-        8066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP-AURKA K162M-mCherry for protein purification in 
            bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M
  TITLE     A FRET biosensor reveals spatiotemporal activation and functions of 
            aurora kinase A in living cells.
  JOURNAL   Nat Commun. 2016 Sep 14;7:12674. doi: 10.1038/ncomms12674.
  PUBMED    27624869
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-14"; volume: "7:12674. doi"; pages: " 
            10.1038/ncomms12674"
COMMENT     Sequence Label: pET21a-GFP-AURKA_K162M-mCherry
FEATURES             Location/Qualifiers
     source          1..8066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     212..231
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     315..334
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        315..333
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    334..358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             373..395
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             403..435
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(445..447,448..450,451..1161)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(445..447,448..450,451..1161)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(490..511)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(751..770)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1098..1119
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2077..2088
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2398..3105
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2543..2561)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2811..2830
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3112..3129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3182..3200)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3196..3243
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3280..3735
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3367..3386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3577..3598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3761..3865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3866..3934,3935..4726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4084..4103)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4897..5485
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5386..5405
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5639..5656
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5671..5810
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5736..5755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5896..5918
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5912..6103)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6912..7994)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALANSLMQLARQVSRLESGQ"
     primer_bind     7956..7975
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(join(7995..8066,1..6))
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
       61 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
      121 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
      181 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
      241 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
      301 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
      361 tctagaaata attttgttta actttaagaa ggagatatac atatggctag catgactggt
      421 ggacagcaaa tgggtcgcgg atccatggtg agcaagggcg aggagctgtt caccggggtg
      481 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      541 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      601 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      661 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      721 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      781 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      841 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      901 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      961 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1021 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1081 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1141 ggcatggacg agctgtacaa ggaattcgag ctcaccatgg accgatctaa agaaaactgc
     1201 atttcaggac ctgttaaggc tacagctcca gttggaggtc caaaacgtgt tctcgtgact
     1261 cagcaatttc cttgtcagaa tccattacct gtaaatagtg gccaggctca gcgggtcttg
     1321 tgtccttcaa attcttccca gcgcgttcct ttgcaagcac aaaaacttgt ctccagtcac
     1381 aagccggttc agaatcagaa gcagaagcaa ttgcaggcaa ccagtgtacc tcatcctgtc
     1441 tccaggccac tgaataacac ccaaaagagc aagcagcccc tgccatcggc acctgaaaat
     1501 aatcctgagg aggaactggc atcaaaacag aaaaatgaag aatcaaaaaa gaggcagtgg
     1561 gctttggaag actttgaaat tggtcgccct ctgggtaaag gaaagtttgg taatgtttat
     1621 ttggcaagag aaaagcaaag caagtttatt ctggctctta tggtgttatt taaagctcag
     1681 ctggagaaag ccggagtgga gcatcagctc agaagagaag tagaaataca gtcccacctt
     1741 cggcatccta atattcttag actgtatggt tatttccatg atgctaccag agtctaccta
     1801 attctggaat atgcaccact tggaacagtt tatagagaac ttcagaaact ttcaaagttt
     1861 gatgagcaga gaactgctac ttatataaca gaattggcaa atgccctgtc ttactgtcat
     1921 tcgaagagag ttattcatag agacattaag ccagagaact tacttcttgg atcagctgga
     1981 gagcttaaaa ttgcagattt tgggtggtca gtacatgctc catcttccag gaggaccact
     2041 ctctgtggca ccctggacta cctgccccct gaaatgattg aaggtcggat gcatgatgag
     2101 aaggtggatc tctggagcct tggagttctt tgctatgaat ttttagttgg gaagcctcct
     2161 tttgaggcaa acacatacca agagacctac aaaagaatat cacgggttga gttcacattc
     2221 cctgactttg taacagaggg agccagggac ctcatttcaa gactgttgaa gcataatccc
     2281 agccagaggc caatgctcag agaagtactt gaacacccct ggatcacagc aaattcatca
     2341 aaaccatcaa attgccaaaa caaagaatca gctagcaaac agtctgtcga caagcttatg
     2401 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     2461 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2521 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     2581 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     2641 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     2701 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     2761 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     2821 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     2881 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     2941 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     3001 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3061 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagctcga gcaccaccac
     3121 caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     3181 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     3241 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
     3301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3481 ccaaaaaact tgattggggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3721 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4381 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     4441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5041 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5641 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     5701 gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5761 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     5821 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5881 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5941 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     6001 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     6061 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     6121 ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac
     6181 gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg
     6241 tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata
     6301 cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa
     6361 tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca
     6421 ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc
     6481 gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca
     6541 acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata atggcctgct
     6601 tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga
     6661 ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc
     6721 cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag
     6781 tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
     6841 aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa cttacattaa
     6901 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6961 gaattggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt
     7021 ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc
     7081 agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac
     7141 ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca
     7201 ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg
     7261 gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa
     7321 ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg
     7381 agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct
     7441 aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg
     7501 tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac
     7561 gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag
     7621 ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct
     7681 tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga
     7741 gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg
     7801 ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc
     7861 agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg
     7921 ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac
     7981 gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata
     8041 ccgcgaaagg ttttgcgcca ttcgat
//
