LOCUS       sequence-19280-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf203
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             397..438
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             448..465
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(488..505)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    494..718
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt cccgaattcc agctgaccac catgctgttc aggatattat
       61 ctctaaattt aataaagatc aaaagacgac gacaccgcca tctacccaac cttctcagac
      121 gttaccaaca actacatgta cacaacaatc ggatggaaat atttcttgta ctactccaac
      181 ggttacacct cctcaacctc ctattgtggc cactgtatgt actcctacac ctactggtgg
      241 tacagtatgt acaacagcac aacaaaatcc aaatccagga gcagcatctc aacaaaatct
      301 aggcaattcc cggggatccg aataaagggt caagacaatt ctgcagatat ccagcacagt
      361 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
      421 ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc
      481 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
      541 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
      601 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
      661 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
      721 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
      781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcg
//
