LOCUS       sequence-19283-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf206
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             464..505
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             515..532
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(555..572)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    561..672
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatgtcatg ttatacagct
       61 atattaaaat ctgtaggagg actggcgcta tttcaagtag ccaatggcgc catagattta
      121 tgtagacatt tctttatgta tttttgtgaa caaaagctac gaccaaattc attttggttc
      181 gttgttgtta gagccattgc gagcatgata atgtatttag tattaggtat agcattgctg
      241 tatatttctg aacaagatga caagaagaat actaataatg ccaacactaa taatgatagt
      301 aatagtaata atagtaacaa tgataaacga aatgagtcgt ctataaattc taactccagt
      361 cctaagtaag caattcccgg ggatccgaat aaagggtcaa gacaattctg cagatatcca
      421 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
      481 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
      541 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
      601 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
      661 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
      721 ggacagcaag ggggaggatt gggaagacaa t
//
