LOCUS       sequence-192846-        5775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 400 / 490, 
            Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5775)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..5775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1266
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    2575..2696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2612..2631)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2666..2685
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2703..3158)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2840..2861)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3052..3071
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3185..3289
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3314..3334)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3358..3568
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3380..3576
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3427..3562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3611..4405
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3611..4405
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3665..3684)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4275..4294
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4593..4612)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4637..4684
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5013..5601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5502..5521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
      601 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
      661 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
      721 tggcccaccc tggtggccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
      781 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
      841 accatcttct tcgaggatga cggcaactac aagacgcgcg ccgaggtgaa gttcgagggc
      901 gataccctgg tgagtcgcat cgagctgacc ggcactgatt tcaaggagga tggcaacatc
      961 ctgggcaata agatggagta caactacaac gccaccaatg tgtacatcgt ggccgacaag
     1021 gccaggaatg gcatcaaggt gaacttcaag gtccgccaca acatcaagga tggcagcgtg
     1081 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     1141 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     1201 cacatgatct acctcgagtt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     1261 tacaagtccg gactcagatc tggcagcggt ggaggcagcg catccggcgg aagcggaagc
     1321 gatgatgata tcgccgcgct cgtcgtcgac aacggctccg gcatgtgcaa ggccggcttc
     1381 gcgggcgacg atgccccccg ggccgtcttc ccctccatcg tggggcgccc caggcaccag
     1441 ggcgtgatgg tgggcatggg tcagaaggat tcctatgtgg gcgacgaggc ccagagcaag
     1501 agaggcatcc tcaccctgaa gtaccccatc gagcacggca tcgtcaccaa ctgggacgac
     1561 atggagaaaa tctggcacca caccttctac aatgagctgc gtgtggctcc cgaggagcac
     1621 cccgtgctgc tgaccgaggc ccccctgaac cccaaggcca accgcgagaa gatgacccag
     1681 atcatgtttg agaccttcaa caccccagcc atgtacgttg ctatccaggc tgtgctatcc
     1741 ctgtacgcct ctggccgtac cactggcatc gtgatggact ccggtgacgg ggtcacccac
     1801 actgtgccca tctacgaggg gtatgccctc ccccatgcca tcctgcgtct ggacctggct
     1861 ggccgggacc tgactgacta cctcatgaag atcctcaccg agcgcggcta cagcttcacc
     1921 accacggccg agcgggaaat cgtgcgtgac attaaggaga agctgtgcta cgtcgccctg
     1981 gacttcgagc aagagatggc cacggctgct tccagctcct ccctggagaa gagctacgag
     2041 ctgcctgacg gccaggtcat caccattggc aatgagcggt tccgctgccc tgaggcactc
     2101 ttccagcctt ccttcctggg catggagtcc tgtggcatcc acgaaactac cttcaactcc
     2161 atcatgaagt gtgacgtgga catccgcaaa gacctgtacg ccaacacagt gctgtctggc
     2221 ggcaccacca tgtaccctgg cattgccgac aggatgcaga aggagatcac tgccctggca
     2281 cccagcacaa tgaagatcaa gatcattgct cctcctgagc gcaagtactc cgtgtggatc
     2341 ggcggctcca tcctggcctc gctgtccacc ttccagcaga tgtggatcag caagcaggag
     2401 tatgacgagt ccggcccctc catcgtccac cgcaaatgct tctagggatc caccggatct
     2461 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2521 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2581 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2641 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     2701 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2761 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2821 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2881 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     2941 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3001 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3061 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3121 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3181 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3241 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     3301 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3361 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3421 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3481 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3541 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     3601 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     3661 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     3721 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     3781 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     3841 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     3901 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     3961 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4021 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4081 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     4141 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4201 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4261 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4321 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4381 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     4441 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4501 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4561 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     4621 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     4681 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     4741 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     4801 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     4861 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4921 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4981 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5041 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5101 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5161 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5221 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5281 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5341 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5401 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5461 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5521 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5581 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5641 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5701 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     5761 atatatggag ttccg
//
