LOCUS       sequence-192855-       11462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SPI1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 11462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-SPI1
FEATURES             Location/Qualifiers
     source          1..11462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          355..558
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     515..535
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    637..670
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(677..1294,1295..1300,1301..1336,1337..1339,1340..1375,
                     1376..1378,1379..1414,1415..1423)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    1476..2050
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1644..1661)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1871..1890
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2066..2068,2069..2071,2072..2785)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2066..2068,2069..2071,2072..2782)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2111..2132)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2372..2391)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2719..2740
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2921..2940
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3228..3816
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3281..3301)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3699..3710)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3891..4124
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4243..4261)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4329..4433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4434..4502,4503..5294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4652..4671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5465..6053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5954..5973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6207..6224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6464..6485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6500..6505,6506..6523,6524..6530)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6538..6554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6543..6565
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6562..6578
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6562..6578
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6628..6647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6839..7472
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7519..7644
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8141..8374
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8559..8603
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8896..9013
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(9068..9086,9087..9109,9110..9128,9129..9151,9152..9170,
                     9171..9193,9194..9212,9213..9235,9236..9254,9255..9277,
                     9278..9296,9297..9319,9320..9338)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9371..9409
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9406..9430
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9539..9563
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10383..10407)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10460..10486
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        10725..10936
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 sgggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
       61 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
      121 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
      181 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
      241 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
      301 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
      361 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
      421 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
      481 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
      541 ttttttcttc catttcaggt gtcgtgaagc ggccgccatg ctagtaaatt gtccgctaaa
      601 ttctggccgt ttttggcttt tttgttagac ggatccataa cttcgtataa tgtatgctat
      661 acgaagttat gccaccatgt ctagactgga caagagcaaa gtcataaacg gagctctgga
      721 attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
      781 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
      841 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
      901 gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga
      961 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     1021 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     1081 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     1141 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     1201 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     1261 tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt
     1321 tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc
     1381 tgacgctctt gacgattttg accttgacat gctccccggg taactaagta aggatccgcg
     1441 gccgcactag aggaattccg cccctctccc ccccccccct ctcccctccc ccccccctaa
     1501 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     1561 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     1621 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     1681 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     1741 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     1801 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     1861 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     1921 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     1981 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     2041 acgatgataa gcttaccggt ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2101 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2161 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2221 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     2281 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2341 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2401 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2461 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2521 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2581 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2641 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     2701 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2761 cggcatggac gagctgtaca agtaagcatg cccgacggcg aggatctcgt cgtgacccat
     2821 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     2881 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     2941 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     3001 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg atgtacaagt
     3061 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3121 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3181 tgtttagtcc ctcccaattc gatatcaagc ttatcgcgat agatcctaat caacctctgg
     3241 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3301 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3361 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     3421 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     3481 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     3541 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     3601 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     3661 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     3721 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     3781 agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac
     3841 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     3901 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     3961 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4021 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4081 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     4141 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg
     4201 gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
     4261 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4321 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4381 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4441 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4501 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4561 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4621 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     4681 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4741 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4801 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4861 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4921 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4981 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5041 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5101 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5161 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5221 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5281 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5341 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5401 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5461 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5521 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5581 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5641 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5701 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5761 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5821 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5881 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5941 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6001 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6061 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6241 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
     6301 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6361 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
     6421 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6481 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6541 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
     6601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6661 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     6721 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     6781 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     6841 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     6901 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     6961 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     7021 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     7081 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     7141 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     7201 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     7261 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     7321 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7381 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7441 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7501 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7561 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7621 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7681 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7741 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7801 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7861 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7921 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7981 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8041 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8101 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8161 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8221 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8281 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8341 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8401 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8461 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8521 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8581 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8641 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8701 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8761 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8821 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc
     8881 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     8941 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     9001 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctaggatcct
     9061 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     9121 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     9181 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     9241 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     9301 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagctcggta
     9361 cccgggtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     9421 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     9481 ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt ggaattctgc agatatcaac
     9541 aagtttgtac aaaaaagcag aagggccgtc aaggcccacc atggaagggt ttcccctcgt
     9601 cccccctcca tcagaagacc tggtgcccta tgacacggat ctataccaac gccaaacgca
     9661 cgagtattac ccctatctca gcagtgatgg ggagagccat agcgaccatt actgggactt
     9721 ccacccccac cacgtgcaca gcgagttcga gagcttcgcc gagaacaact tcacggagct
     9781 ccagagcgtg cagcccccgc agctgcagca gctctaccgc cacatggagc tggagcagat
     9841 gcacgtcctc gataccccca tggtgccacc ccatcccagt cttggccacc aggtctccta
     9901 cctgccccgg atgtgcctcc agtacccatc cctgtcccca gcccagccca gctcagatga
     9961 ggaggagggc gagcggcaga gccccccact ggaggtgtct gacggcgagg cggatggcct
    10021 ggagcccggg cctgggctcc tgcctgggga gacaggcagc aagaagaaga tccgcctgta
    10081 ccagttcctg ttggacctgc tccgcagcgg cgacatgaag gacagcatct ggtgggtgga
    10141 caaggacaag ggcaccttcc agttctcgtc caagcacaag gaggcgctgg cgcaccgctg
    10201 gggcatccag aagggcaacc gcaagaagat gacctaccag aagatggcgc gcgcgctgcg
    10261 caactacggc aagacgggcg aggtcaagaa ggtgaagaag aagctcacct accagttcag
    10321 cggcgaagtg ctgggccgcg ggggcctggc cgagcggcgc cacccgcccc acggcctcat
    10381 gggcccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct
    10441 agagggcccg cggttcgaat acccatacga cgtcccagac tacgcttagt aatgattaat
    10501 taaactagaa attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagtaaccc
    10561 acccaagatc tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
    10621 ggcgagccgc gtaagatctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
    10681 ctcctcacgg cgagcctagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc
    10741 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
    10801 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
    10861 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
    10921 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
    10981 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
    11041 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
    11101 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
    11161 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
    11221 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
    11281 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
    11341 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
    11401 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
    11461 gg
//
