LOCUS       sequence-19286-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf209
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             408..449
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             459..476
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(499..516)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    505..729
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgagctcgga tccgtaccct tcaccgaatt ccagctgacc accatggaca tgcttatgat
       61 tggaaattat ttttccggcg tgctaatcgc tggaatcatt cttttgattc tttcgtgtat
      121 cttcgccttt attgacttta gtaagtctac cagtcccact cgtacatgga aagtattgag
      181 tattatggcg tttatacttg gtattattat cacagtcgga atgctaattt attctatgtg
      241 gggaaagcac tgcgcgcccc acagagttag cggagtcatt cataccaatc atagcgatat
      301 ttccatgaac taagcaattc ccggggatcc gaataaaggg tcaagacaat tctgcagata
      361 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
      421 ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag
      481 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
      541 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
      601 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
      661 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
      721 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
      781 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      841 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc cttcttt
//
