LOCUS       sequence-192867-       11630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SOX10 gRNA used for targeted demethylation is inserted into the WPXL
            lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V,
            Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, 
            Huangfu D
  TITLE     TET proteins safeguard bivalent promoters from de novo methylation 
            in human embryonic stem cells.
  JOURNAL   Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. 
            Epub 2017 Dec 4.
  PUBMED    29203910
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-017-0002-y"; journalName: "Nat Genet"; date: 
            "2018-01"; volume: "50"; issue: "1"; pages: "83-95"
COMMENT     Sequence Label: WPXL SOX10 gRNA
FEATURES             Location/Qualifiers
     source          1..11630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2120..3297
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2351..3288
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3245..3265
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3326..3373
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3392..4417
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3392..4417
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    4531..4652
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4681..4798
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4888..4912
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4929..5177
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4929..4949
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5100..5119
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5199..5274
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(5392..5416)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5460..6048
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5513..5533)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5931..5942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6192..6225)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6245..6425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6450..6468)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6451..6468)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6758..6777)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6991..7010)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7110..7132
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7170..7188)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7256..7360
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7361..7429,7430..8221)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7579..7598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8392..8980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8881..8900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9134..9151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9221..9241)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9226..9555
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9406..9541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10689..10754
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10884..10904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11329..11463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatgtcgac gataagcttt
     2041 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2101 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2161 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     2221 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2281 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2341 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     2401 cccttgcgtg ccttgaatta cttccactgg ctgcagtacg tgattcttga tcccgagctt
     2461 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     2521 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     2581 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     2641 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     2701 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     2761 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     2821 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     2881 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     2941 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     3001 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     3061 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     3121 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     3181 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     3241 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga
     3301 atttcgacat ttaaatttaa ttaatgttga caattaatca tcggcatagt atatcggcat
     3361 agtataatac gacaaggtga ggaactaaac catgaaaaag cctgaactca ccgcgacgtc
     3421 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     3481 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     3541 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     3601 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     3661 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     3721 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     3781 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     3841 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     3901 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     3961 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     4021 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     4081 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     4141 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     4201 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     4261 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     4321 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     4381 ccgacgcccc agcactcgtc cgagggcaaa ggaatagtct agaggatcat aatcagccat
     4441 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     4501 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     4561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4621 tgtggtttgt ccaaactcat caatgtatct tattaattaa tctcgacggt atcggttaac
     4681 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4741 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat
     4801 cgatcacgag actagcctcg aggtttaaac tacgggatcc aggcctaagc ttacgcgtcc
     4861 cagtgtggtg gaattctgca gatatcaaca agtttgtaca aaaaagcagg ctccgcggtg
     4921 gcggccgcga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     4981 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     5041 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     5101 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     5161 ggaaaggacg aaacaccgca cagtaagaga gacttctcgt tttagagcta gaaatagcaa
     5221 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     5281 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca
     5341 attctgcaga caaatggctc tagaggtacg aattcaaggg tgggcgcgcc gacccagctt
     5401 tcttgtacaa agtggttgat atccagcaca gtggtcgacg ataaaactag tcatatgata
     5461 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5521 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5581 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5641 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5701 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5761 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5821 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5881 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5941 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6001 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cggtacgtat
     6061 ggccaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     6121 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tgggatcaat
     6181 tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc tttttgcttg
     6241 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6301 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6361 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6421 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     6481 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     6541 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     6601 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     6661 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     6721 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     6781 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     6841 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     6901 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     6961 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7021 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     7081 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     7141 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     7201 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     7261 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7321 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7381 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7441 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7501 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7561 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7621 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7681 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7741 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7801 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7861 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7921 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7981 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8041 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8101 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8161 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8221 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8281 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8341 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8401 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8461 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8521 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8581 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8641 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8701 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8761 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     8821 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8881 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8941 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9001 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9061 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9121 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9181 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
     9241 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9301 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9361 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9421 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9481 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9541 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
     9601 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     9661 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     9721 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
     9781 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
     9841 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
     9901 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
     9961 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
    10021 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
    10081 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
    10141 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
    10201 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
    10261 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
    10321 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
    10381 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
    10441 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
    10501 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
    10561 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
    10621 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
    10681 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
    10741 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
    10801 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
    10861 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
    10921 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
    10981 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
    11041 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
    11101 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
    11161 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
    11221 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
    11281 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    11341 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11401 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    11461 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    11521 accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    11581 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
//
