LOCUS       sequence-19287-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf210
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             405..446
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             456..473
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(496..513)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    502..613
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tcaccgaatt ccagctgacc accatggttc gtagacgata attcgttgat aatttattct
       61 acatggccca gtacatttgt ccgattcatc aggtagggtc atcgttatgc cagataatag
      121 atcattcacg tttaaggaag ggtttaaatt agatgaatcg ataaaatcta tattgttggt
      181 aaacccgtcg tctatagatc tattaaagat tagagtatat aaacatcgca taaaatggat
      241 gggtgatata ttcgtattat ttgagcaaga aaatatccca ccaccttttc gtctagtaaa
      301 tgataagtaa gcaattcccg gggatccgaa taaagggtca agacaattct gcagatatcc
      361 agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta
      421 accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt
      481 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      541 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
      601 aggaaattgc atcgcattgt ctgagtaggt gtcattc
//
