LOCUS       sequence-192879-        2425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 8a of EMMA. Functional category: 
            IRES sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1760 HC_Kan_IRES2_p8b
FEATURES             Location/Qualifiers
     source          1..2425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(112..686,687..689,690..698)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(279..296)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     506..525
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     terminator      770..801
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        802..904
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             905..1720
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(978..997)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1741..1768
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1780..2367)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1860..1879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2389..2418)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat
       61 tcgttacgtc tcgatcccgc tcgataagct tgatatcgaa ttccgccccc ccccctctcc
      121 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
      181 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
      241 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
      301 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
      361 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
      421 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
      481 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
      541 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
      601 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
      661 tttcctttga aaaacacgat gataatatgg ccacaaccgc gtcgagacga attctctaga
      721 tatcgctcaa tactgaccat ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc
      781 tccgaccgga ggcttttgac ttgatcggca cgtaagaggt tccaactttc accataatga
      841 aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc
      901 taaaatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat
      961 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     1021 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
     1081 tagcgttgcc aatgatgtta cagatgagat ggtcaggcta aactggctga cggaatttat
     1141 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     1201 tgcgatccca gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     1261 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     1321 tccttttaac ggcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     1381 tttggttggt gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     1441 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt
     1501 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     1561 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     1621 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     1681 taaattgcag tttcacttga tgctcgatga gtttttctga gggcccaaat gtaatcacct
     1741 ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt ttccataggc tccgcccccc
     1801 tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga caggactata
     1861 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1921 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1981 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2041 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2101 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2161 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2221 aacagtattt ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag agttggtagc
     2281 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2341 attacgcgca gaaaaaaagg atctcaagaa gatcctttga ttttctaccg aagaaaggcc
     2401 cacccgtgaa ggtgagccag tgagt
//
