LOCUS       sequence-192881-        1866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 8a of EMMA. Functional category: 
            p2A sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1883 HC_Kan_p2A_p8
FEATURES             Location/Qualifiers
     source          1..1866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      16..47
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        48..150
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             151..966
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(224..243)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      987..1014
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1026..1613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1106..1125)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1635..1664)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(1749..1805)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga tcggcacgta
       61 agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta
      121 tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga aacgtcttgc
      181 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
      241 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
      301 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
      361 aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
      421 cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt ccaggtatta
      481 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
      541 ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt tcgtctcgct
      601 caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga tgacgagcgt
      661 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg
      721 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
      781 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
      841 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
      901 tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct cgatgagttt
      961 ttctgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg
     1021 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag
     1081 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1141 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1201 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1261 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1321 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1381 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1441 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1501 gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1621 ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag ttgattgcag
     1681 tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa ttcgtttcgt
     1741 ctcgacgcag gtccagggtt ctcctccacg tcgccagcct gcttcagcag gctgaagtta
     1801 gtagcgcctc gagacgtgac gaattctcta gatatcgctc aatactgacc atttaaatca
     1861 tacctg
//
