LOCUS       sequence-192893-        2410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 9 of EMMA. Functional category: 
            Selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1899 HC_Kan_PuroR_p9
FEATURES             Location/Qualifiers
     source          1..2410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      32..63
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        64..166
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             167..982
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(240..259)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1003..1030
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1042..1629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1122..1141)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1651..1680)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(1767..2363)
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(1850..1870)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 atttaaatca tacctgacct ccatagcaga aagtcaaaag cctccgaccg gaggcttttg
       61 acttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
      121 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatga gccatattca
      181 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
      241 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
      301 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
      361 tacagatgag atggtcaggc taaactggct gacggaattt atgcctcttc cgaccatcaa
      421 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc cagggaaaac
      481 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
      541 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acggcgatcg
      601 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg gtgcgagtga
      661 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
      721 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
      781 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
      841 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
      901 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcactt
      961 gatgctcgat gagtttttct gagggcccaa atgtaatcac ctggctcacc ttcgggtggg
     1021 cctttctgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1081 tcgatgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1441 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1501 cgctctgctg aagccagtta cctcggaaaa agagttggta gctcttgatc cggcaaacaa
     1561 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1621 ggatctcaag aagatccttt gattttctac cgaagaaagg cccacccgtg aaggtgagcc
     1681 agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa tgatatcaag
     1741 cttgaattcg ttcgtctcga gcattattag gcaccgggct tgcgggtcat gcaccaggtc
     1801 gcgcggtcct tcgggcactc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     1861 gggaggttgc ggggcgcgga ggtttccagg aaggcgggca ccccggcgcg ctcggccgcc
     1921 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgacacg
     1981 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     2041 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     2101 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     2161 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     2221 agctcggtga cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag
     2281 tcggcgaacg cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg
     2341 cgcaccgtgg gcttgtactc ggtggacgcc gagacgtgac gaattctcta gatatcgctc
     2401 aatactgacc
//
