LOCUS       sequence-192895-        1842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 6 of EMMA. Functional category: 
            Signal peptide for subcellular targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1903 HC_Kan_SV40-NLS_p6
FEATURES             Location/Qualifiers
     source          1..1842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             87..107
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      185..216
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        217..319
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             320..1135
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(393..412)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1156..1183
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1195..1782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1275..1294)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1804..1833)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 attgcagtcc agttacgctg gagtctgagg ctcgtcctga atgatatcaa gcttgaattc
       61 gttacgtctc gccagccgcc accatgccta agaagaaacg gaaagtgggc agccgagacg
      121 gacgaattct ctagatatcg ctcaatactg accatttaaa tcatacctga cctccatagc
      181 agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa
      241 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      301 aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct cgaggccgcg
      361 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      421 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
      481 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca ggctaaactg
      541 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      601 atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc
      661 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
      721 tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc
      781 acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta atggctggcc
      841 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt
      901 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
      961 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1021 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1081 taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt tctgagggcc
     1141 caaatgtaat cacctggctc accttcgggt gggcctttct gcgttgctgg cgtttttcca
     1201 taggctccgc ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga ggtggcgaaa
     1261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1621 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttacctcgga
     1681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgattttc
     1801 taccgaagaa aggcccaccc gtgaaggtga gccagtgagt tg
//
