LOCUS       sequence-192897-        1948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 22 of EMMA. Functional category: 
            Insulator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1911 HC_Kan_Insulator_FB_p2
FEATURES             Location/Qualifiers
     source          1..1948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      292..323
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        324..426
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             427..1242
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(500..519)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1263..1290
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1302..1889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1382..1401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1911..1940)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt
       61 cgttacgtct cgatggggcc gcgaattctg aaagacccca cctgtaggtt tggcaagccc
      121 agggatgtac gtccctaacc cgctaggggg cagcaactag tcccaggcct gcactgccgc
      181 ctgccggcag gggtccagtc gctagcgcat gcctgcagac tcgagacgac gaattctcta
      241 gatatcgctc aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag
      301 cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat
      361 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
      421 gctaaaatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
      481 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
      541 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
      601 ggtagcgttg ccaatgatgt tacagatgag atggtcaggc taaactggct gacggaattt
      661 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
      721 actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
      781 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
      841 tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
      901 ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
      961 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1021 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     1081 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     1141 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     1201 aataaattgc agtttcactt gatgctcgat gagtttttct gagggcccaa atgtaatcac
     1261 ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc
     1321 cctgacgagc atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta
     1381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1741 agaacagtat ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta
     1801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg
     1921 cccacccgtg aaggtgagcc agtgagtt
//
