LOCUS       sequence-192905-        7088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is for expression of a rhodopsin/guanylyl cyclase 
            fusion protein. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Trieu MM, Devine EL, Lamarche LB, Ammerman AE, Greco JA, Birge RR, 
            Theobald DL, Oprian DD
  TITLE     Expression, purification, and spectral tuning of RhoGC, a 
            retinylidene/guanylyl cyclase fusion protein and optogenetics tool 
            from the aquatic fungus Blastocladiella emersonii.
  JOURNAL   J Biol Chem. 2017 Jun 23;292(25):10379-10389. doi: 
            10.1074/jbc.M117.789636. Epub 2017 May 4.
  PUBMED    28473465
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.789636"; journalName: "J Biol Chem"; date: 
            "2017-06-23- 23"; volume: "292"; issue: "25"; pages: "10379-10389"
COMMENT     Sequence Label: pMT3-RhoGC
FEATURES             Location/Qualifiers
     source          1..7088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          446..675
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     658..677
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2784..3347
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    3534..3668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3571..3590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3625..3644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4341..4358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4512..5100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4592..4611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5271..6062,6063..6131))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5894..5913
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6132..6236)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6304..6322
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6360..6382)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6482..6501
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6695..6717
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6709..6726
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6710..6726
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6736..7065)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6750..6885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7050..7070
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 aatttcgagc ggtgttccgc ggtcctcctc gtatagaaac tcggaccact ctgagacgaa
       61 ggctcgcgtc caggccagca cgaaggaggc taagtgggag gggtagcggt cgttgtccac
      121 tagggggtcc actcgctcca gggtgtgaag acacatgtcg ccctcttcgg catcaaggaa
      181 ggtgattggt ttataggtgt aggccacgtg accgggtgtt cctgaagggg ggctataaaa
      241 gggggtgggg gcgcgttcgt cctcactctc ttccgcatcg ctgtctgcga gggccagctg
      301 ttgggctcgc ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac
      361 ccgtcggcct ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg
      421 atcggaaaac ctctcgactg ttggggtgag tactccctct caaaagcggg catgacttct
      481 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg
      541 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc
      601 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg
      661 cctttctctc cacaggtgtc cactcccagg tccaactgca agaattccac catgccgagg
      721 ggacccgaca ggcccgaagg aatcgaagaa gccaaagaca aagacaataa tctgcgcggc
      781 gcctgcagta gctgcaactg tccggaatat tgctttagcc cgaccagtac cctgtgcgac
      841 gattgtaagt gcagcgtgac caaacatccg attgttgagc agccgctgag tcgcaatggt
      901 agctttcgca gtagtggtgc aagcctgctg ccgagcccga gcagtccgaa tgtgaaaatt
      961 accagcaccg tgggtctgcg cagtcgcaag agcgagagcc aggccaatgt gcgcggcagc
     1021 atgattagca acagcaatag cggcagccgt agtaacaaca gtggtggcgc cggtggtggt
     1081 agtggcggta gcagtagcag caaaggcggc agcgcattag caaattatca gagcgcaatg
     1141 agcgaactgt ggagctggaa catgatgctg agcaccccga gtctgaagtt tctgaccgtt
     1201 cagtttacca cctggattgt tctgaccacc gttggcgcca tctacaccct gttcttccat
     1261 gagcgtcagg cctacaaccg tggctgggcc gatatttggt acggctatgg cgcctttggc
     1321 ttcggcctgg gcctgagttt tgcctatatg ggctttaccg gcgcacgcaa ccctgaaaag
     1381 aaggccctga gcttatgcct gctgggtgtt aatttcatta gctttatgag ttatatcatt
     1441 atcatgttac gcctgacccc gaccatcgaa ggtactatgg ccaatccggt ggaaccggca
     1501 cgctatctgg aatggatcgc cacctgcccg gtgctgattc tgctgattag tgagatcacc
     1561 cagtatccgc acgacccgta taaagtgatc gtgaacgatt acgcactgtg cctggcaggt
     1621 ttcgtgggtg caatcagcgc acagcagccg tggggcgatc tggcccattt tgtgagctgc
     1681 ctgtgcttca gctacgtggt gtatagcctg tggagttgct ttacaggtgc catcgatggc
     1741 gaaacccagt gcaatgtgga aaaaagcggc ctgcgctgga ttcgttttag caccatcacc
     1801 acctggagcc tgtttcctat cacctggttc agctacacca gtggtctgat tagttttacc
     1861 gtggcagagg caggcttcag catgattgac attggcgcca aagtgtttct gacactggtg
     1921 ctggtgaata gcaccgtgga gcaagcccag aatcaaaagg tggacgccat taccgccatt
     1981 gccgaggagc tggagaatca gattaataac tgtgatgcaa tcctgcaaaa aatgatgccg
     2041 gaaggcgtgt tagagcagct gaagaatggt caggccaccg aagccaaaga atacgaaagc
     2101 gttaccgtgt tcttcagtga catcaccaac tttaccgtga ttagcagccg taccagcacc
     2161 aaggatatga tggccaccct gaacaagtta tggctggagt atgatgcaat cgccaagcgc
     2221 tggggtgtgt ataaggtgga gacaatcggc gacgcatatc tgggtgttac cggtgcaccg
     2281 gatgtggttc cggatcatgc agagcgcgcc tgcaattttg ccgtggacat cattgagatg
     2341 atcaaaagct ttaagacaat taccggcgaa agcattaaca ttcgtattgg cttaaatagc
     2401 ggcccggtga ccgcaggcgt gttaggtgat ctgaacccgc attggtgcct ggttggtgac
     2461 accgttaata ccgccagtcg tatggagagc accagtaaag ccggccacat tcatattagt
     2521 gagagcacct atcattttat taaaagcaaa ttcgtgaccc agccgctgga cgtgatggaa
     2581 gtgaagggca aaggcaagat gcagacatac tgggttctgg gtcgcaaaat gcatggcagc
     2641 ggcagcgcag agaccagcca agtggcgcct gcctaagcgg ccgcaaattc cggggggggg
     2701 gacagctcag ggctgcgatt tcgcgccaaa cttgacggca atcctagcgt gaaggctggt
     2761 aggattttat ccccgctgcc atcatggttc gaccattgaa ctgcatcgtc gccgtgtccc
     2821 aaaatatggg gattggcaag aacggagacc taccctggcc tccgctcagg aacgagttca
     2881 agtacttcca aagaatgacc acaacctctt cagtggaagg taaacagaat ctggtgatta
     2941 tgggtaggaa aacctggttc tccattcctg agaagaatcg acctttaaag gacagaatta
     3001 atatagttct cagtagagaa ctcaaagaac caccacgagg agctcatttt cttgccaaaa
     3061 gtttggatga tgccttaaga cttattgaac aaccggaatt ggcaagtaaa gtagacatgg
     3121 tttggatagt cggaggcagt tctgtttacc aggaagccat gaatcaacca ggccacctca
     3181 gactctttgt gacaaggatc atgcaggaat ttgaaagtga cacgtttttc ccagaaattg
     3241 atttggggaa atataaactt ctcccagaat acccaggcgt cctctctgag gtccaggagg
     3301 aaaaaggcat caagtataag tttgaagtct acgagaagaa agactaacag gaagatgctt
     3361 tcaagttctc tgctcccctc ctaaagctat gcatttttat aagaccatgg gacttttgct
     3421 ggctttagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3481 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3661 tctggatccc cggccaacgg tctggtgacc cggctgcgag agctcggtgt acctgagacg
     3721 cgagtaagcc cttgagtcaa agacgtagtc gttgcaagtc cgcaccaggt actgatatcc
     3781 caccaaaaag tgcggcggcg gctggcggta gaggggccag cgtagggtgg ccggggctcc
     3841 gggggcgagg tcttccaaca taaggcgatg atatccgtag atgtacctgg acatccaggt
     3901 gatgccggcg gcggtggtgg aggcgcgcgg aaagtcgcgg acgcggttcc agatgttgcg
     3961 cagcggcaaa aagtgctcca tggtcgggac gctctggccg gtgaggcgtg cgcagtcgtt
     4021 gacgctctag accgtgcaaa aggagagcct gtaagcgggc actcttccgt ggtctggtgg
     4081 ataaattcgc aagggtatca tggcggacga ccggggttcg aaccccggat ccggccgtcc
     4141 gccgtgatcc atgcggttac cgcccgcgtg tcgaacccag gtgtgcgacg tcagacaacg
     4201 ggggagcgct ccttttggct tccttccagg cgcggcggct gctgcgctag cttttttggc
     4261 gagctcgaat taattctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4321 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4381 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4441 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4501 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4561 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4621 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4681 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4741 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4801 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4861 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4921 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4981 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5041 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5101 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5161 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5221 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5281 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5341 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5401 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5461 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5521 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5581 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5641 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5701 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5761 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5821 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5881 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5941 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6001 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6061 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6121 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6181 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6241 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     6301 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     6361 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6421 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     6481 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     6541 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     6601 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     6661 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     6721 gccagtgcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     6781 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     6841 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     6901 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     6961 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     7021 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acaggatccg
     7081 gtcgcgcg
//
