LOCUS       sequence-192908-        2521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 9 of EMMA. Functional category: 
            Fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2034 HC_Kan_mTagBFP2_p9
FEATURES             Location/Qualifiers
     source          1..2521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      24..55
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        56..158
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             159..974
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(232..251)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      995..1022
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1034..1621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1114..1133)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1643..1672)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(1759..2466)
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDM
                     ALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
ORIGIN
        1 catacctgac ctccatagca gaaagtcaaa agcctccgac cggaggcttt tgacttgatc
       61 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
      121 ttgagttatc gagattttca ggagctaagg aagctaaaat gagccatatt caacgggaaa
      181 cgtcttgctc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
      241 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
      301 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
      361 agatggtcag gctaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
      421 tccgtactcc tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc
      481 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
      541 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacggcgat cgcgtatttc
      601 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tggtgcgagt gattttgatg
      661 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat
      721 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
      781 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
      841 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
      901 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcac ttgatgctcg
      961 atgagttttt ctgagggccc aaatgtaatc acctggctca ccttcgggtg ggcctttctg
     1021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgatgct
     1081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1201 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1441 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1501 tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1621 agaagatcct ttgattttct accgaagaaa ggcccacccg tgaaggtgag ccagtgagtt
     1681 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt
     1741 cgttcgtctc gagcattaat taagcttgtg ccccagtttg ctagggaggt cgcagtatct
     1801 ggccactgcc acctcgtgct gctcgacgta ggtttcgttg ttggcctcct tgattctttc
     1861 cagtctgtag tccacatagt agacgccagg catcttgagg ttcttagcgg gtttcttgga
     1921 tctatatgtg gtcttggcgt ttgcgatcag atggctcccg cccacgagct tcagggccat
     1981 gtcgtttctg ccttccaggc cgccgtcagc ggggtacagc gtttcggtga aggcctccca
     2041 gccgagtgtt ttcttctgca tcacagggcc gttggatgtg aagttcaccc ctctgatctt
     2101 gacgttgtag atgaggcagc cgtcctggag gctggtgtcc tgggtagcgg tcagcacgcc
     2161 cccgtcttcg tatgtggtga ctctctccca tgtgaagccc tcagggaagg actgcttgaa
     2221 gaagtcgggg atgccctggg tgtggttgat gaaggtcttg ctgccgtaga ggaagctagt
     2281 agccaggatg tcgaaggcga aggggagagg gccgccctcg accaccttga ttctcatggt
     2341 ctgggtgccc tcgtagggct tgccttcgcc ctcggatgtg cacttgaagt gatggttgtc
     2401 cacggtgccc tccatgtaca gcttcatgtg catgttctcc ttaatcagct cttcgccctt
     2461 agacacggac gccgagacgt gacgaattct ctagatatcg ctcaatactg accatttaaa
     2521 t
//
