LOCUS       sequence-192916-        2096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 9 of EMMA. Functional category: 
            Dimerization domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2052 HC_Kan_DmrC_p9
FEATURES             Location/Qualifiers
     source          1..2096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      36..67
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        68..170
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             171..986
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(244..263)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1007..1034
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1046..1633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1126..1145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1655..1684)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(1775..2053)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 gaccatttaa atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct
       61 tttgacttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
      121 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata
      181 ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat
      241 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
      301 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      361 atgttacaga tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca
      421 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atcccaggga
      481 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
      541 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg
      601 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga
      661 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
      721 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
      781 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
      841 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
      901 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
      961 acttgatgct cgatgagttt ttctgagggc ccaaatgtaa tcacctggct caccttcggg
     1021 tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1081 aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1501 tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa
     1561 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1621 aaaaggatct caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg
     1681 agccagtgag ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat
     1741 caagcttgaa ttcgttcgtc tcgagcagtt attactttga gattcgtcgg aacacatgat
     1801 aatagaggtc ccaggcttgg aggaggtcct tgacattccc tgatttcatg tacttcctgc
     1861 accactcttg ggcctccatt aaatctcgac cataggcctg attaaaggat gtttccttca
     1921 gagtctgggg gccccgttcc atcatagcat gcaagggctc cagcacctca aacatgcctt
     1981 tcacgttcct ttccccaaag tacaaacgag atgcctcttc caggccttca tgccacatct
     2041 catgccagag gatggacgcc gagacgtgac gaattctcta gatatcgctc aatact
//
