LOCUS       sequence-19292-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf215
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             359..400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             410..427
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(450..467)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    456..663
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctcggat ccgtaccctt caccgaattc cagctgacca ccatgttatt aactaacgtt
       61 ataaaatctg cttctggata tcccaaacat tctctgctta aatcaaatgc tgattggttt
      121 tttatttttc gttttacagt tccggattta ttaatctcat caactaactc atcgacagtt
      181 gcgcactcat taatatctaa tttattagtc aattgcttta agcctgcact accaacctta
      241 gagaaatact ttccgttaac ataagcaatt cccggggatc cgaataaagg gtcaagacaa
      301 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
      361 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
      421 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      481 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      541 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
      601 gggtggggtg gggcaggaca gcaaggggga ggattgggaa cacaatatca ggcatgctgg
      661 gga
//
