LOCUS       sequence-192921-        3032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 18 of EMMA. Functional category: 
            Constitutional promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2741 HC_Kan_EF1ap_p18
FEATURES             Location/Qualifiers
     source          1..3032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        75..1253
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          306..1244
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1201..1221
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     terminator      1375..1406
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1407..1509
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1510..2325
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1583..1602)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2346..2373
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2385..2972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2465..2484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2994..3023)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 attgcagtcc agttacgctg gagtctgagg ctcgtcctga atgatatcaa gcttgaattc
       61 gtacgtctcg cggtggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
      121 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
      181 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
      241 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
      301 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
      361 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
      421 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
      481 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
      541 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
      601 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
      661 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
      721 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
      781 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
      841 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
      901 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
      961 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     1021 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     1081 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     1141 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     1201 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgacgctagc
     1261 gctaccggac tcagatctcg agctcaagct tcgaattcgt gccgagacga aacgaattct
     1321 ctagatatcg ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa
     1381 aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat
     1441 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     1501 gaagctaaaa tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc
     1561 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1621 gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1681 aaaggtagcg ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa
     1741 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     1801 accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt
     1861 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     1921 aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     1981 aacggtttgg ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     2041 gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt cactcatggt
     2101 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     2161 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     2221 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     2281 atgaataaat tgcagtttca cttgatgctc gatgagtttt tctgagggcc caaatgtaat
     2341 cacctggctc accttcgggt gggcctttct gcgttgctgg cgtttttcca taggctccgc
     2401 ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga
     2461 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2521 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2581 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2641 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2701 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2761 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2821 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttacctcgga aaaagagttg
     2881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2941 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgattttc taccgaagaa
     3001 aggcccaccc gtgaaggtga gccagtgagt tg
//
