LOCUS       sequence-192923-        1860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 8 of EMMA. Functional category: 
            Peptide linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe2746 HC_Kan_Linker1_p8
FEATURES             Location/Qualifiers
     source          1..1860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      35..66
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        67..169
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             170..985
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(243..262)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1006..1033
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1045..1632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1125..1144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1654..1683)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga ccggaggctt
       61 ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
      121 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tgagccatat
      181 tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata
      241 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
      301 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      361 tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc ttccgaccat
      421 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa
      481 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
      541 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacggcga
      601 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag
      661 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
      721 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
      781 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
      841 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
      901 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
      961 cttgatgctc gatgagtttt tctgagggcc caaatgtaat cacctggctc accttcgggt
     1021 gggcctttct gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1081 aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1501 ctgcgctctg ctgaagccag ttacctcgga aaaagagttg gtagctcttg atccggcaaa
     1561 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1621 aaaggatctc aagaagatcc tttgattttc taccgaagaa aggcccaccc gtgaaggtga
     1681 gccagtgagt tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc
     1741 aagcttgaat tcgtttcgtc tcgacgctcc gcttccgcca gatgcgctgc ctcctccgct
     1801 gccagatctg agtccagagc ctcgagacgt gacgaattct ctagatatcg ctcaatactg
//
