LOCUS       sequence-192928-        2728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 10 of EMMA. Functional category: 
            Long Terminal Repeat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3766 HC_Kan_3'-LTR_p10
FEATURES             Location/Qualifiers
     source          1..2728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    86..674
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(139..159)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(557..568)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             746..978
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     terminator      1072..1103
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1104..1206
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1207..2022
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1280..1299)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2043..2070
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2082..2669)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2162..2181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2691..2720)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt
       61 cgtacgtctc gtgctttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
      121 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
      181 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
      241 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
      301 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
      361 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
      421 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
      481 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
      541 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
      601 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
      661 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
      721 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
      781 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      841 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      901 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc ttttagtcag
      961 tgtggaaaat ctctagcagg gcccgtttaa acccgcggta cgagacggac gaattctcta
     1021 gatatcgctc aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag
     1081 cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat
     1141 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     1201 gctaaaatga gccatattca acgggaaacg tcttgctcga ggccgcgatt aaattccaac
     1261 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     1321 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     1381 ggtagcgttg ccaatgatgt tacagatgag atggtcaggc taaactggct gacggaattt
     1441 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     1501 actgcgatcc cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     1561 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     1621 tgtcctttta acggcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     1681 ggtttggttg gtgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     1741 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     1801 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     1861 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     1921 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     1981 aataaattgc agtttcactt gatgctcgat gagtttttct gagggcccaa atgtaatcac
     2041 ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc
     2101 cctgacgagc atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta
     2161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2521 agaacagtat ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta
     2581 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2641 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg
     2701 cccacccgtg aaggtgagcc agtgagtt
//
