LOCUS       sequence-19293-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf216
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             347..388
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             398..415
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(438..455)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    444..668
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gtacccttca ccgaattcca gctgaccacc atggatgttt cgttactact aggaaaacag
       61 acaggtcggt tttctccctt attgttgtac ggcttagcag aatatgcggc tgttaaaata
      121 acctctatca acatagatat agtttttcta gagaagatgg atgtagaatt aataatgaca
      181 tctggtgtga taccattttc gtcgtatgga agttccgttt catccgcgac atacgcgact
      241 gttccctttt gagcaattcc cggggatccg aataaagggt caagacaatt ctgcagatat
      301 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
      361 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
      421 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
      481 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
      541 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
      601 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
      661 ctctatgggc tctgaggcgg aaagaaccag ctggggctct agggggtatc cc
//
