LOCUS       sequence-192934-        2493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part designed to occupy position 3 of EMMA. Functional category: 
            Constitutional promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe1759 HC_Kan_CAGp_p3
FEATURES             Location/Qualifiers
     source          1..2493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        96..475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..752
                     /label=chicken beta-actin promoter
     terminator      839..870
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        871..973
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             974..1789
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1047..1066)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1810..1837
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1849..2436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1929..1948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2458..2487)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa
       61 ttcgtacgtc tcggactgga tctgatatca tcgtcgacat tgattattga ctagttatta
      121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
      481 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
      541 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg
      601 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
      661 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
      721 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgttggga ccgagacgaa
      781 ttctctagat atcgctcaat actgaccatt taaatcatac ctgacctcca tagcagaaag
      841 tcaaaagcct ccgaccggag gcttttgact tgatcggcac gtaagaggtt ccaactttca
      901 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
      961 taaggaagct aaaatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa
     1021 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     1081 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     1141 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcaggctaa actggctgac
     1201 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     1261 actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc
     1321 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     1381 ttgtaattgt ccttttaacg gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     1441 gaataacggt ttggttggtg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1501 acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca
     1561 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     1621 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     1681 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     1741 tgatatgaat aaattgcagt ttcacttgat gctcgatgag tttttctgag ggcccaaatg
     1801 taatcacctg gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt tccataggct
     1861 ccgcccccct gacgagcatc acaaaaatcg atgctcaagt cagaggtggc gaaacccgac
     1921 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1981 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2041 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2101 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2161 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2221 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2281 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct cggaaaaaga
     2341 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2401 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat tttctaccga
     2461 agaaaggccc acccgtgaag gtgagccagt gag
//
