LOCUS       sequence-192940-       10843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Halo-GFP for neuronal inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Han X, Boyden ES
  TITLE     Multiple-color optical activation, silencing, and desynchronization 
            of neural activity, with single-spike temporal resolution.
  JOURNAL   PLoS One. 2007 Mar 21;2(3):e299.
  PUBMED    17375185
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2007-03-21"; volume: "2(3)"; pages: "e299"
COMMENT     Sequence Label: FCK-Halo-GFP
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     160..180
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(189..617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(299..320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     511..530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    663..887
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     876..893
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             919..1099
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1604..1620
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1605..1621
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1624..2212
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1730..1741
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2139..2159
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(2237..2950,2951..2953))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(2240..2950,2951..2953))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2282..2303)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2631..2650
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2890..2911
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3857..5145)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    5190..5307
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5605..5649)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5834..6067
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6560..6685
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6732..6912
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6930..7128)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6955..6975)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7130..7509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7681..7700
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7775..7879
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7880..7948,7949..8740)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8098..8117)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8911..9499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9400..9419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9653..9670
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9787..9808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9823..9828,9829..9846,9847..9853)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9861..9877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9866..9888
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9885..9901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9885..9901
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9950..10071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9961..9980)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10015..10034
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(10201..10575)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(10594..10641)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(10689..10843,1..175))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10703..10838
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10757..10776)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
       61 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
      121 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
      181 acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
      241 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
      301 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
      361 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
      421 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
      481 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
      541 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
      601 gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga
      661 agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt
      721 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
      781 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
      841 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
      901 gcgggtttaa acgggccctg ctagagattt tccacactga ctaaaagggt ctgagggatc
      961 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     1021 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     1081 ggtctaacca gagagaccca gtacagtccg gatgcagctc tcgggccatg tgatgaaatg
     1141 ctaggcggct gtcaaacctc cactctaata cttctctctc cgggtcatcc atcccatgca
     1201 ggctcacagg gtgtaacaag cgggtgttct ctccttcatt ggcttcttct accttctctt
     1261 gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg atccctggcc
     1321 ctggtgtgta gttctgccaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa
     1381 ggatatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa
     1441 agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc tgaggtgtga
     1501 ctggaaaacc cacctcctcc tcctcttgtg cttctagcca ggcacaatca gcattggtag
     1561 ctgctgtatt gctacttgtg attgctccat gtttttctag gtctcgaggt cgacggtatc
     1621 gatgcgggga ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg
     1681 gaagaggccg cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg
     1741 aagggacgta gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc
     1801 aaggaaagga cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag
     1861 cccctgtcca gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg
     1921 ggaaagcgaa agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg
     1981 ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct
     2041 cataaagaga cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag
     2101 caatagcatg atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat
     2161 agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa
     2221 gcttgatatc gaattcttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     2281 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
     2341 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     2401 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     2461 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     2521 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     2581 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     2641 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     2701 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     2761 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg caaatgaact
     2821 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     2881 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     2941 cgcccttgct cactgctact accggtcggt catcggcagg tgtgccgctg gcggatggca
     3001 cgtccagtat ggagccggac accacggact cattggaagt cagataattc agcagcagaa
     3061 aggcaaaaat atacttggcc acaatgtcca ggaaggagta gccccagctt gtcacaccaa
     3121 caggcagaac tgcgattccc tccacgccga gtgcccaaac gatagggtaa cccagccaca
     3181 tcaccacagt gagcagcttc agtgtattaa acatgtcggc tgtccctgca gccttggcat
     3241 cctgagccca ctccaccagc aggatataca gcaccaccag aaagcaggca caagagatgg
     3301 cataccaaaa ccatctcatc agatggctgc tggtggtgag ggcagctgcg agcccggtca
     3361 cgcacatggc gatatcgaat gtaatagcag tgaagagttt ggtggcgtta cttccggcca
     3421 ggagtccgag tgccagcagg atcatggggg tactgagagc ccaggtgagg tagcgacccc
     3481 acattgtgac aaccccatcg acctcttcgc ctcccagcat gacagaactc ccttcggcaa
     3541 agtggccagc tggcatttcc aggacactga ttgtgagacc agatgccagc ccagtgtagg
     3601 aagcgataga gaccacggga accagaattg tgctcactgc aatcagttta gccctagggt
     3661 catcgagtcc cctggtcatg aagacgaaca gcagtataga caggcctgcc agagcaatgt
     3721 tgatgtagag gctagaagcc aggagggggt cgttgaggac gaactcgaac agctcccgct
     3781 gggtaacctc tgcttgcaga gcgacggcgc tttcagtcac gggtgggagg gtctcagtca
     3841 tggtggcgga tcccccgctg cccccagaac taggggccac tcgcctgccc gtgctcctga
     3901 gtgcaaacgg agaaccggct tgactgacga gcttggggct tctgagcagg gcactgtggc
     3961 tgctcacagc ctcgcgagcc ttcgtcagca tccaggtccc catggcaacc acctccgaaa
     4021 cgccctgttc ccgttgcccc ggtaactgcc tccccaacac ctgcctgcct ttcactttaa
     4081 aacctgctcc tctttgccct ggccttatat acacaatact gcaggactgt gtgggcccag
     4141 gagcaagtgg accctgttcc cccagggtga tgtactgagg gggttggaga aggggatgcg
     4201 aacaaactta gtccacaagt gatcatcgct cttcctacct cccccacctc caactatagg
     4261 gcccttttaa cagacgggat gggattaagt ggggcaaaga ggcagttgct atggtaacgg
     4321 ctagtgggtg ccacattctg ggatggtcct tgagaaagac aggatgtagt taccctggca
     4381 acttcatctc ctctgaagag acaggtattg ccccgttgcc ctggcaacag ctgggttcta
     4441 aggtttacag gggaagctaa ttcatctctc cttcccttcc cccttgtagt ctctgtaatg
     4501 gtttctttgc ttcctctggg aattggtctg gttgacttgg cctctgtctc tgtccttgtt
     4561 tctttccagc tctccctgct ttttagccat tgtgcatatc acaccgccac cttagcgtga
     4621 gaagaagtac caaacagacc cagaatgctg acggaatgac tctgtctgct ttctcacccc
     4681 tatctgtcac cactgagcag cagggcccct cctgaggcct ctgccacagc tctccccacc
     4741 catcctccca gagggaccat gaagatttgg tctcctgggg ctcctgtccc agtttctgct
     4801 tgtgcttccc gtcaaggtag gactggagag aggcaactat aatgaggtga acgtggctgg
     4861 actcacccag ggctgggcgg gtggtgtaga tgtaccctgc cctggactct tctttcctaa
     4921 actcattggt catctgccct aatgagttca ctccttctgt atatcgtact tgcatgcccc
     4981 caaggcatgg aatttaagat tagtaatctg agttcaagtt ctagctctga catttctgat
     5041 gtgaccctgg gcaggtcact gaggaagctc tctgcacccc catctcattt tctagaaaat
     5101 cagaagaaga accccatgga gatgaagtga cctaaggcca taatgttaat taaccaaact
     5161 ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
     5221 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     5281 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg tctgcagaat
     5341 tggcgcacgc agtgccgatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
     5401 accttcttct tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg
     5461 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
     5521 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     5581 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     5641 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     5701 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     5761 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     5821 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     5881 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     5941 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     6001 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     6061 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     6121 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     6181 gtccctcata tctcctcctc caggtctgaa gatcagcggc cgcttgctgt gcggtggtct
     6241 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
     6301 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
     6361 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
     6421 ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt
     6481 ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa
     6541 ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag
     6601 tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag
     6661 caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt
     6721 tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     6781 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     6841 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     6901 ccagagagac ccagtacagg caaaacgcgc tgcttatata gacctcccac cgtacacgcc
     6961 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     7021 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     7081 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     7141 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     7201 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     7261 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     7321 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     7381 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     7441 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     7501 atcaatgtca acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
     7561 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
     7621 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
     7681 cggcatcaga gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc
     7741 gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7801 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7861 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7921 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7981 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8041 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8101 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8161 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8221 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8281 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8341 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8401 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8461 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8521 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8581 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8641 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8701 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8761 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8821 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8881 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8941 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9001 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9061 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9121 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9181 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9241 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9301 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9361 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9421 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9481 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9541 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9601 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9661 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9721 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9781 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9841 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9901 ccatgattac gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt
     9961 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
    10021 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc
    10081 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
    10141 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg
    10201 tcagtcctgc tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa
    10261 ctcccgcccc cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa
    10321 gttcgtggac acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac
    10381 ccaggccagg gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac
    10441 gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg
    10501 gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact
    10561 ggtcaacttg gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta
    10621 tgccgatgat taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc
    10681 gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
    10741 tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
    10801 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcg
//
