LOCUS       sequence-192942-        9197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is a Cryptococcus neoformans mCherry expression vector with 
            G418 drug selection marker and C. neoformans SH2 flanking sequences 
            for genome integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Upadhya R, Lam WC, Maybruck B, Donlin MJ, Chang AL, Kayode S, 
            Ormerod KL, Fraser JA, Doering TL, Lodge JK
  TITLE     A fluorogenic C. neoformans reporter strain with a robust expression
            of m-cherry expressed from a safe haven site in the genome.
  JOURNAL   Fungal Genet Biol. 2017 Sep 1. pii: S1087-1845(17)30136-6. doi: 
            10.1016/j.fgb.2017.08.008.
  PUBMED    28870457
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2017 Sep 1. pii: S1087-1845(17)30136-6. doi: 
            10.1016/j.fgb.2017.08.008."
COMMENT     Sequence Label: pH3mCHSH2
FEATURES             Location/Qualifiers
     source          1..9197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(467..500)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(971..1681)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVRLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1249..1268)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1518..1536
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3165..3174
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3169..3178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3208..3227)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3209..3227)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3234..3251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3234..3250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3243..3265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3391..3846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3478..3497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3688..3709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3924..4028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4029..4097,4098..4889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4247..4266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5060..5648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5549..5568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5802..5819
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5936..5957
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5972..5977,5978..5995,5996..6002)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6010..6026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6015..6037
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6034..6050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6034..6050
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6068..6086
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6068..6085
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(7041..7074)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(8035..8835)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSMIEQDGLHAGSPAAWVERLFSYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8146..8165)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 atgcagatca gcgtggggag agcaccatct agcagcccct ggcaggccca gcattgtgct
       61 cggatgtgcg ataggcggtg ggtgcgataa ggtggattag tggaggatgg aggactgagt
      121 ggcgggcaag ggaagggggc gggaagatga ctcaccttgg gcagtgggtg ggggatgcgg
      181 tataggggat gtatgtggat gctggcggag gatagaagct gtaagctgag ctggtgccaa
      241 cggtgacgct gtgagagtgg tctcggcgac cctgacggcg actcggcgtt accccgtgtg
      301 gcacaccgga cgcgcgtcga gtgacgttgc tgctattgtt gttaccatca tcctctcctc
      361 cgacaaccat acactcagcg cacaccatgc cactcgaatc ctgcatgctt atgtgagtcc
      421 tccccttccc gtgccccgct ctccagctca catccttcta gacctaataa cttcgtatag
      481 catacattat acgaagttat attaagggtt gtcgaaaggg cgaattccag cacactggcg
      541 gccgttacta ctagtgattt gactgaaaaa aggagtgacg gattaaggaa gacgtgaccg
      601 actaaaggaa aggaaggatg gaaggaacga tatcaatgcg atggtggcga cctgagaatg
      661 agtaaatagt tgaaagcact aaaataaagc agcaacgaag gttattccaa gttatagctc
      721 tcgtgcaaat ttcaacagtg atggccattt tattgttgca agagacgtcg tcagccgaga
      781 aatattcgat ttcgcggaca actatcggga atccaataac ggacaaacga catggaatga
      841 tatatcacgg caatgaagat tttgaagcgc agaggtatac atcagcaaat aagacacaaa
      901 acgtaagaag cacaagtagc aacagtcatc cgatgcatat tacgcgtaag aagggtcact
      961 acataagtaa ctacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
     1021 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
     1081 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     1141 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
     1201 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
     1261 ttacggggcc gtcggagggg aagttggtgc cgcgcagcct caccttgtag atgaactcgc
     1321 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
     1381 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
     1441 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
     1501 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     1561 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
     1621 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
     1681 ttgtgataaa tgtactgagg tggttgttgc tggtttggaa gaagagtaga taagagtggg
     1741 aaaagacttg tttgcggttg ctgatgagaa gtgggaatat ctcgagggcc aagggtttat
     1801 atattggccg agatatcctc ccgcaggccg tgttcaccag gagcgagtgt ttgtttatct
     1861 cggagaatag gcattacggc gttatagaaa atccataatt cgattattca aagaaagtca
     1921 cgtgacttaa tggataaatc atttcctccc tctgtaattc cctcgtaaat aaatcgaagt
     1981 gaaaatgaat tttcccaggc accggtattt tccataacgc cgatttgtat tatacgcggc
     2041 ttgtaatccc atctttccat catcccttct tcccacccag aacactgtcc atcaaacaaa
     2101 catccatgta tccaaatatt gccagcatat caatttatct aggccatccc ggggcagttt
     2161 tcgagtcttc tcatcgataa tcgaattccc caaaatcgtt gaacccgcac atttttttct
     2221 aatgccgagc atggaatggc tgaaccatgc gcaagacagc atgatagcat gacagcatta
     2281 tttcatctag taaatgcttg tattacctta tgctctgagt acttggacct atttatgagc
     2341 tactagaaat gagagcgtgc atttgattaa cagataaaaa tagctgtggc aacttggcgt
     2401 atttattgcg gcgcaacgtc tgtagtttga gtaaagagca gttgtgcaaa tacagaagat
     2461 tacttgctgc aagcaaagca ccgcccggca aattctcttc tactctctca tttctctcta
     2521 tgcccacgct gcaagagctc atagcaggta agcgtttgct cgggaacaaa gacaaaaaat
     2581 atgagacagt ccgggaaaat acccatgcaa gcacggaaaa gagagcctga ggtaggaata
     2641 aatcgtccct taatccaggt acaggttggc atcttatggt atcattttat tctatcatac
     2701 agaacttcct acttttggct tcattaatcc tattacggat cacttacgac tcattcctat
     2761 gccgttcaac agcattatga cagaatcaat catggaaaat tatgtcaagg atgcatttca
     2821 gacaattgta gagaccgtac acagacaaga gaaaagagaa gaaaagaacg aagaagggag
     2881 gcgacagccg actaatccct ccgcgcttgc cggggacgat agctcccgtt cttctttggc
     2941 ccttctttgc ataatattgc catatgcttt ctgcctccca ttagattact gagatgtcta
     3001 tcacccaaga ctccacagcc taagattaaa gtgattcccc gtgaccgaca gcgcaccctt
     3061 ttcgggttta atatcacatg ataaagacca gctctagact ccaaactttt atttaagaca
     3121 tcagcttcag ccgctgtacg ccatccgcag gcctgaaaca acgggccgcc atggcggccg
     3181 ggagcatgcg acgtcgggcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     3241 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     3301 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3361 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     3421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3601 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3721 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3781 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3841 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatcag
     3901 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3961 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4021 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4081 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4141 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4201 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4261 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4321 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4381 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4441 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4501 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4561 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4621 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4681 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4741 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4801 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4861 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4921 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4981 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5041 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5101 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5161 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5221 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5281 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5341 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5401 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5461 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5521 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5581 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5641 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5701 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5761 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5821 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5881 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5941 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     6001 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     6061 ccaagctatt taggtgacac tatagaatac tcaagctatg catccaacgc gttgggagct
     6121 ctcccatatg gtcgacctgc aggcggccgc gaattcacta gtccttcatc aacattctga
     6181 cgctttgttt cgtgaagctt gtctcgttta attttatttc cgggcctttc ggcacaactc
     6241 ttttttaaat ttgatcggca agaaagaggg tgctgtctca tcttctcgtg ttcggatctt
     6301 ccacctaacc tccacttttg gttttatacc ttgacacttg acaacccgcc tttcctccat
     6361 cttccaccct cctttactac cgttcaagca ccgaaagcat tgtcgtcctt attgcctttg
     6421 aattacatgg agtagttgca aggcccaaca tctgaagatg atggaaggcc ttcgtcgccc
     6481 atattttact aattacagcc tgccgctcgc cggtgcatgc tgtctgctgc caggagcatg
     6541 ttaagtgttg aatcatgatg agttctgaga ccgttcatta ccttctttgc cttacagatc
     6601 atccttttgc atctattatt acatatttgc tcggaatcta gcacaatcta cctttatttt
     6661 agtggtttgt gcaattccct ttgagcgata agtaaaaagt gaggaacaag aggcgatgca
     6721 agctacgagt atagaacgtt accgaagagg aataacgaga gacaaaatga tcaaagcacc
     6781 cagatcgatc aagatccagg aagttacctc aaccatcact cattcgttat gtgcaccact
     6841 acgtgaatga caattgcgaa accgaaaaaa ataaatatcg ttcagcactc agtactgaaa
     6901 gcatcggagc ttagaatagc tcgagcaata gcagctgact acctgctgaa gaccgaagaa
     6961 caagggaagg ccaacggaag accttgaatc ttagacccgc cagtgtgatg gatatctgca
     7021 gaattcgccc ttactaccta ataacttcgt atagcataca ttatacgaag ttatattaag
     7081 ggttctcgag agctggggga cagatgatat ccgacaagag ggtgaagagg gaggaaagca
     7141 ggactctggt gattctgggg ttttgagagt cccgaaccgg ttgggaaaac caaaaaggga
     7201 tagcgatgga aaaagtttaa aaaaagtgga agaagaggta gatggaaatg aggatgaaaa
     7261 aggaggagaa gaagaagaag aagatggaaa agttgtggag aaggaagaga ctagaagcaa
     7321 ggggaaacgg aggcataaac aaactcacaa gcatggtaca gagagcgacc aaactcgtcc
     7381 cgtggacgaa aaatccaaaa gaaaagaaag cacgcacaaa aggcccaaaa atagacaccg
     7441 ccgctcctct gcctcctcct cgtccaagaa gaagaaaaag agacttcata aagaagacga
     7501 gctagaatcg ccgtccacgc catggtttga aattgccttt gaagggcttc cttttggacc
     7561 agatgtaccc gcggtaagtc tgattcccat ctgctgccat tttccattcg aatgctttga
     7621 tcgtcgtttc cgggataaag ggcgctgacg cgatttgaat tttgggcgtg atcaatccca
     7681 gttcaagatg ctcggctgga tgaggaaaca gtggagactc ttgttggcag aggcggtgtt
     7741 cattcttggg ttagtgtaca tcattcaagc tacaggactg taaaatcaga ctccttctac
     7801 ctttttttcc ttggaatgtt ttacattgtg tatgtccatg tttgcaggtt cttgtctccc
     7861 gttgtaaatg ggaaattaca attagtgtcg agtgacactt ttgtacttgg gcaaaatatg
     7921 catgtcatag cacagcgtta gcaatgtatc aagaatgtca agaagactgt ctatccgctt
     7981 gttgcggtcg agagaaattg agagaatgaa gagacggggt ttgaggggga attctcagaa
     8041 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     8101 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     8161 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     8221 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     8281 atcctcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc
     8341 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     8401 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     8461 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     8521 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     8581 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     8641 ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     8701 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     8761 gtcatagctg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccgtcttg
     8821 ttcaatcatg gatccgggcg agtttactaa tggaaaaaga caattagcaa agaggctgca
     8881 tattaggaga cgcggtgaag attggggagg acaatagggt ccgtgcacca aaaagtcctg
     8941 acggcagctc tgttcaacgc acatccatct cttccagcta tgcgtgtggc accagcacgg
     9001 atggattgtt gcaccaaaag gacaaggcga gatggatgca ggtcgaggcg atgcggtgct
     9061 atcggccctc gttgtcgaac gccacgtcgt cctctcggcg accgtcaatc ctggaaggaa
     9121 atgaccaagc gtgcacccag catccagcat ccaacatcca gcacccagca cctagcaccc
     9181 atgatccaat ggccggc
//
