LOCUS       sequence-19295-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf218
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             822..863
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             873..890
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgagctcgga tccgtaccct tcaccgaatt ccagctgacc accatgaaac caatgccgaa
       61 acagagagag atgcgacgtc ttagagatag aatctcagat attgaaagac agttgagtga
      121 ctgcagacgt aataatgaaa gcaatgctga tatggaaaga gagatgcaac gtcttagaga
      181 tagaatcatg gatcttgata gacagcttaa cgagtgtaaa cgcaacggta acggaacatc
      241 ttctgaggag gtaaataggc taaagactag aatcaggaat cttaaacgat cgctagagat
      301 ctgctcaaag gatgaatcag aactctattc agcatataaa actaaactcg gacgtgctag
      361 ggaacaaatt agtaacttgc aagaaagtct acgtagagag cgtgaatctg acaaaacaga
      421 tagttactac aggagggaat tgactcgcga aagaaataaa atcgtagaaa tggaaaaaga
      481 acttaataag tgtttcgatg ccaagtacat cgacgaaatc aattccaaga aaacccgtat
      541 ttctgatctc gaacgacaac tagcagcctg taaatctaat ggtggtagca atggaaacat
      601 ggaccaatac aaacgggaaa ttgaatctct taaacgcgag ctcgccgagt gtagacgtgg
      661 taacaatgga tcccatagtg attgtaagta ctatgacgaa gaagcaagag attgtgttaa
      721 gagctgagca attcccgggg atccgaataa agggtcaaga caattctgca gatatccagc
      781 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
      841 ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tg
//
