LOCUS       sequence-192962-       13025 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary construct of plasma membrane-targeted sfGFP1-10 for plant 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13025)
  AUTHORS   Park E, Lee HY, Woo J, Choi D, Dinesh-Kumar SP
  TITLE     Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type
            III Secretion Using Split Fluorescent Protein Fragments.
  JOURNAL   Plant Cell. 2017 Jul;29(7):1571-1584. doi: 10.1105/tpc.17.00047. 
            Epub 2017 Jun 14.
  PUBMED    28619883
REFERENCE   2  (bases 1 to 13025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00047"; journalName: "Plant Cell"; date: "2017-07"; 
            volume: "29"; issue: "7"; pages: "1571-1584"
COMMENT     Sequence Label: PEP108E
FEATURES             Location/Qualifiers
     source          1..13025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      58..310
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    332..356
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1657..2286
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2715..3788
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3854..4048
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4284..4306)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4392..4532
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4447..4466
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4547..4564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4718..5306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4798..4817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6731..7525)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7950..7974
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8052..8226
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8267..9292)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9359..10035)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(9402..9422)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(12401..12429)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat
       61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
      121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
      181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
      241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
      301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
      361 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
      421 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
      481 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
      541 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
      601 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
      661 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
      721 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
      781 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
      841 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
      901 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
      961 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
     1021 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
     1081 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
     1141 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
     1201 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
     1261 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
     1321 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
     1381 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
     1441 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
     1501 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
     1561 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
     1621 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
     1681 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
     1741 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
     1801 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
     1861 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
     1921 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
     1981 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
     2041 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
     2101 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
     2161 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
     2221 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
     2281 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
     2341 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
     2401 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
     2461 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
     2521 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
     2581 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
     2641 tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag
     2701 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     2761 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     2821 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     2881 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     2941 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     3001 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     3061 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     3121 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     3181 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     3241 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     3301 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     3361 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     3421 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     3481 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     3541 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     3601 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     3661 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     3721 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     3781 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     3841 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     3901 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     3961 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     4021 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     4081 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     4141 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     4201 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     4261 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     4321 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     4381 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     4441 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     4501 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     4561 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4621 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4681 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4741 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4801 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4861 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4921 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4981 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5041 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5101 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     5161 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5221 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5281 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5341 ctcagtggaa cgaaaactca cgttaagggc tgatgaatcc cctaatgatt ttggtaaaaa
     5401 tcattaagtt aaggtggata cacatcttgt catatgatca aatggtttcg cgaaaaatca
     5461 ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt accaattctg
     5521 ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg cattacttga
     5581 ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag cgagaacaaa
     5641 acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctccaca aagagcgact
     5701 cgctgtatac cgttggcatg ctagctttat ctgttcgggc aatacgatgc ccattgtact
     5761 tgttgactgg tctgatattc gtgagcaaaa acgacttatg gtattgcgag cttcagtcgc
     5821 actacacggt cgttctgtta ctctttatga gaaagcgttc ccgctttcag agcaatgttc
     5881 aaagaaagct catgaccaat ttctagccga ccttgcgagc attctaccga gtaacaccac
     5941 accgctcatt gtcagtgatg ctggctttaa agtgccatgg tataaatccg ttgagaagct
     6001 gggttggtac tggttaagtc gagtaagagg aaaagtacaa tatgcagacc taggagcgga
     6061 aaactggaaa cctatcagca acttacatga tatgtcatct agtcactcaa agactttagg
     6121 ctataagagg ctgactaaaa gcaatccaat ctcatgccaa attctattgt ataaatctcg
     6181 ctctaaaggc cgaaaaaatc agcgctcgac acggactcat tgtcaccacc cgtcacctaa
     6241 aatctactca gcgtcggcaa aggagccatg ggttctagca actaacttac ctgttgaaat
     6301 tcgaacaccc aaacaacttg ttaatatcta ttcgaagcga atgcagattg aagaaacctt
     6361 ccgagacttg aaaagtcctg cctacggact aggcctacgc catagccgaa cgagcagctc
     6421 agagcgtttt gatatcatgc tgctaatcgc cctgatgctt caactaacat gttggcttgc
     6481 gggcgttcat gctcagaaac aaggttggga caagcacttc caggctaaca cagtcagaaa
     6541 tcgaaacgta ctctcaacag ttcgcttagg catggaagtt ttgcggcatt ctggctacac
     6601 aataacaagg gaagacttac tcgtggctgc aaccctacta gctcaaaatt tattcacaca
     6661 tggttacgct ttggggaaat tatgagggga tctctcagcg ttaagggatt ttggtcatgc
     6721 attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
     6781 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
     6841 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
     6901 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
     6961 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
     7021 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
     7081 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
     7141 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
     7201 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
     7261 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
     7321 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
     7381 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
     7441 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
     7501 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
     7561 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
     7621 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
     7681 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
     7741 ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt
     7801 gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc
     7861 ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc
     7921 tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg
     7981 acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     8041 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
     8101 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     8161 gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca cattattatg
     8221 gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactctat ttctttgccc
     8281 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     8341 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     8401 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     8461 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagt cgtggcgatc
     8521 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     8581 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     8641 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     8701 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     8761 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     8821 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     8881 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatag
     8941 cctccgcgac cggttgtaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     9001 caccctgtga acggcgggag atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa
     9061 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     9121 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     9181 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     9241 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatctcatt
     9301 gccccccgga tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc
     9361 agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat
     9421 agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg
     9481 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     9541 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     9601 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     9661 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     9721 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     9781 tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     9841 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     9901 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     9961 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
    10021 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
    10081 tagacgagag tgtcgtgctc caccatgttg ggcccggcgc gccgaattcc cggggatcca
    10141 ctagtaacgg ccgccagtgt gctggaattc aggatcagaa gcttgatcag gatattcttg
    10201 tttaagatgt tgaactctat ggaggtttgt atgaactgat gatctaggac cggataagtt
    10261 cccttcttca tagcgaactt attcaaagaa tgttttgtgt atcattcttg ttacattgtt
    10321 attaatgaaa aaatattatt ggtcattgga ctgaacacga gtgttaaata tggaccaggc
    10381 cccaaataag atccattgat atatgaatta aataacaaga ataaatcgag tcaccaaacc
    10441 acttgccttt tttaacgaga cttgttcacc aacttgatac aaaagtcatt atcctatgca
    10501 aatcaataat catacaaaaa tatccaataa cactaaaaaa ttaaaagaaa tggataattt
    10561 cacaatatgt tatacgataa agaagttact tttccaagaa attcactgat tttataagcc
    10621 cacttgcatt agataaatgg caaaaaaaaa caaaaaggaa aagaaataaa gcacgaagaa
    10681 ttctagaaaa tacgaaatac gcttcaatgc agtgggaccc acggttcaat tattgccaat
    10741 tttcagctcc accgtatatt taaaaaataa aacgataatg ctaaaaaaat ataaatcgta
    10801 acgatcgtta aatctcaacg gctggatctt atgacgaccg ttagaaattg tggttgtcga
    10861 cgagtcagta ataaacggcg tcaaagtggt tgcagccggc acacacgagt cgtgtttatc
    10921 aactcaaagc acaaatactt ttcctcaacc taaaaataag gcaattagcc aaaaacaact
    10981 ttgcgtgtaa acaacgctca atacacgtgt cattttatta ttagctattg cttcaccgcc
    11041 ttagctttct cgtgacctag tcgtcctcgt cttttcttct tcttcttcta taaaacaata
    11101 cccaaagagc tcttcttctt cacaattcag atttcaattt ctcaaaatct taaaaacttt
    11161 ctctcaattc tctctaccgt gatcaaggta aatttctgtg ttccttattc tctcaaaatc
    11221 ttcgattttg ttttcgttcg atcccaattt cgtatatgtt ctttggttta gattctgtta
    11281 atcttagatc gaagacgatt ttctgggttt gatcgttaga tatcatctta attctcgatt
    11341 agggtttcat aaatatcatc cgatttgttc aaataatttg agttttgtcg aataattact
    11401 cttcgatttg tgatttctat ctagatctgg tgttagtttc tagtttgtgc gatcgaattt
    11461 gtcgattaat ctgagttttt ctgattaaca gatgctcgac ctgcagatgg caaaggatgt
    11521 ggaagccgtt cccggagaag gatttcagac aagagactat caagatccgc caccagctcc
    11581 gtttattgat ggagcggagc taaagaagtg gtctttctac agagcagtta tcgcagagtt
    11641 cgtagccact ctcctcttct tatacatcac cgttttgaca gtcatcggtt acaagattca
    11701 gtccgatact gatgccggtg gcgtagattg cggcggagtt ggaatcctcg gtatcgcttg
    11761 ggcctttggt ggtatgatct tcatcctcgt ctactgcacc gccggtatct ctggtggtca
    11821 cattaaccca gcggtgacat ttgggctatt cttggcacgt aaagtgtcgt tacctagggc
    11881 cctattgtac ataatcgctc agtgtttggg tgcgatttgt ggagttggtt ttgtcaaagc
    11941 cttccaaagc tcttactaca cccgttacgg aggtggagcc aactctctag ccgatggcta
    12001 cagcacaggg accggtctag ccgcagagat cattggtact ttcgttcttg tctacaccgt
    12061 cttctctgcc actgacccca aacgtagtgc cagagactcc cacgttccgg tgttggcgcc
    12121 acttccaatc ggatttgccg tgttcatggt acatttggct accattccca ttaccggaac
    12181 cggaattaac ccggcaagga gtttcggagc tgccgtaatc tacaacaaga gcaagccatg
    12241 ggatgaccac tggatatttt gggttggacc attcattgga gctgcgatag ctgcattcta
    12301 ccaccaattc gttctaagag cttcaggttc taagtctctt ggatcattca gaagtgctgc
    12361 caacgtccag ctgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
    12421 attagatggt gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc
    12481 tacaattgga aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
    12541 gccaacactt gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca
    12601 catgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac
    12661 tatatctttc aaagatgacg ggaaatacaa gacgcgtgct gtcgtcaagt ttgaaggtga
    12721 tacccttgtt aatcgtatcg agttaaaagg tactgatttt aaagaagatg gaaacattct
    12781 cggacacaaa ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca
    12841 aaagaatgga atcaaagcta acttcaccgt tcgccacaac gttgaagatg gttccgttca
    12901 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
    12961 caaccattac ctgtcgacac aaactgtcct ttcgaaagat cccaacgaaa agtgagttaa
    13021 caacg
//
